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• W2108428329 abstract "A RecA–single-stranded DNA (RecA–ssDNA) filament searches a genome for sequence homology by rapidly binding and unbinding double-stranded DNA (dsDNA) until homology is found. We demonstrate that pulling on the opposite termini (3′ and 5′) of one of the two DNA strands in a dsDNA molecule stabilizes the normally unstable binding of that dsDNA to non-homologous RecA–ssDNA filaments, whereas pulling on the two 3′, the two 5′, or all four termini does not. We propose that the ‘outgoing’ strand in the dsDNA is extended by strong DNA–protein contacts, whereas the ‘complementary’ strand is extended by the tension on the base pairs that connect the ‘complementary’ strand to the ‘outgoing’ strand. The stress resulting from different levels of tension on its constitutive strands causes rapid dsDNA unbinding unless sufficient homology is present." @default.
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• W2108428329 date "2011-10-19" @default.
• W2108428329 modified "2023-10-14" @default.
• W2108428329 title "RecA homology search is promoted by mechanical stress along the scanned duplex DNA" @default.
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• W2108428329 doi "https://doi.org/10.1093/nar/gkr855" @default.
• W2108428329 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3287184" @default.
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