FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108452826> ?p ?o ?g. }

• W2108452826 endingPage "1436" @default.
• W2108452826 startingPage "1429" @default.
• W2108452826 abstract "PAY genes are required for peroxisome assembly in the yeast Yarrowia lipolytica. Here we show that a mutant strain, pay2, is disrupted for the import of proteins targeted by either peroxisomal targeting signal-1 or −2. Electron microscopy of pay2 cells revealed the presence of small peroxisomal “ghosts,” similar to the vesicular structures found in fibroblasts of patients with the human peroxisome assembly disorder, Zellweger syndrome. Functional complementation of pay2 with a plasmid library of Y. lipolytica genomic DNA identified a gene, PAY2, that restores growth of pay2 on oleic acid, import of catalase and multifunctional enzyme into peroxisomes, and formation of wild type peroxisomes. The PAY2 gene encodes Pay2p, a hydrophobic polypeptide of 404 amino acids. An antibody raised against Pay2p recognizes a polypeptide of ∼42-kDa whose synthesis is induced by growth of Y. lipolytica on oleic acid. Pay2p is a peroxisomal integral membrane protein, as it localizes to carbonate-stripped peroxisomal membranes. Pay2p shows no identity to any known protein. Our results suggest that Pay2p is essential for the activity of the peroxisomal import machinery but does not affect the initial steps of peroxisomal membrane proliferation. PAY genes are required for peroxisome assembly in the yeast Yarrowia lipolytica. Here we show that a mutant strain, pay2, is disrupted for the import of proteins targeted by either peroxisomal targeting signal-1 or −2. Electron microscopy of pay2 cells revealed the presence of small peroxisomal “ghosts,” similar to the vesicular structures found in fibroblasts of patients with the human peroxisome assembly disorder, Zellweger syndrome. Functional complementation of pay2 with a plasmid library of Y. lipolytica genomic DNA identified a gene, PAY2, that restores growth of pay2 on oleic acid, import of catalase and multifunctional enzyme into peroxisomes, and formation of wild type peroxisomes. The PAY2 gene encodes Pay2p, a hydrophobic polypeptide of 404 amino acids. An antibody raised against Pay2p recognizes a polypeptide of ∼42-kDa whose synthesis is induced by growth of Y. lipolytica on oleic acid. Pay2p is a peroxisomal integral membrane protein, as it localizes to carbonate-stripped peroxisomal membranes. Pay2p shows no identity to any known protein. Our results suggest that Pay2p is essential for the activity of the peroxisomal import machinery but does not affect the initial steps of peroxisomal membrane proliferation." @default.
• W2108452826 created "2016-06-24" @default.
• W2108452826 creator A5000851275 @default.
• W2108452826 creator A5001047555 @default.
• W2108452826 creator A5003978275 @default.
• W2108452826 creator A5004302932 @default.
• W2108452826 creator A5035253921 @default.
• W2108452826 creator A5080858056 @default.
• W2108452826 date "1995-01-01" @default.
• W2108452826 modified "2023-09-30" @default.
• W2108452826 title "The Yarrowia lipolytica Gene PAY2 Encodes a 42-kDa Peroxisomal Integral Membrane Protein Essential for Matrix Protein Import and Peroxisome Enlargement but Not for Peroxisome Membrane Proliferation" @default.
• W2108452826 cites W1497958401 @default.
• W2108452826 cites W1563473939 @default.
• W2108452826 cites W1567048655 @default.
• W2108452826 cites W1570204140 @default.
• W2108452826 cites W1582080277 @default.
• W2108452826 cites W1645771708 @default.
• W2108452826 cites W1659602917 @default.
• W2108452826 cites W1668858601 @default.
• W2108452826 cites W1741319179 @default.
• W2108452826 cites W1776917887 @default.
• W2108452826 cites W1966799030 @default.
• W2108452826 cites W1975304761 @default.
• W2108452826 cites W1977166690 @default.
• W2108452826 cites W1984636211 @default.
• W2108452826 cites W1986492446 @default.
• W2108452826 cites W1987765816 @default.
• W2108452826 cites W1997312301 @default.
• W2108452826 cites W1998419036 @default.
• W2108452826 cites W2000826918 @default.
• W2108452826 cites W2003228141 @default.
• W2108452826 cites W2005411425 @default.
• W2108452826 cites W2006643920 @default.
• W2108452826 cites W2013206953 @default.
• W2108452826 cites W2015644140 @default.
• W2108452826 cites W201706246 @default.
• W2108452826 cites W2026582002 @default.
• W2108452826 cites W2027647762 @default.
• W2108452826 cites W2030078973 @default.
• W2108452826 cites W2034484654 @default.
• W2108452826 cites W2050038698 @default.
• W2108452826 cites W2053196299 @default.
• W2108452826 cites W2056530947 @default.
• W2108452826 cites W2057579672 @default.
• W2108452826 cites W2062341760 @default.
• W2108452826 cites W2066861084 @default.
• W2108452826 cites W2083543206 @default.
• W2108452826 cites W2084938165 @default.
• W2108452826 cites W2087344385 @default.
• W2108452826 cites W2100837269 @default.
• W2108452826 cites W2102071276 @default.
• W2108452826 cites W2111792196 @default.
• W2108452826 cites W2111817213 @default.
• W2108452826 cites W2114625295 @default.
• W2108452826 cites W2117403951 @default.
• W2108452826 cites W2127006597 @default.
• W2108452826 cites W2127838671 @default.
• W2108452826 cites W2132762612 @default.
• W2108452826 cites W2138270253 @default.
• W2108452826 cites W2142870234 @default.
• W2108452826 cites W2149933948 @default.
• W2108452826 cites W2152478788 @default.
• W2108452826 cites W2155632056 @default.
• W2108452826 cites W2160107586 @default.
• W2108452826 cites W2161127497 @default.
• W2108452826 cites W2167428799 @default.
• W2108452826 cites W2169940566 @default.
• W2108452826 cites W2170566884 @default.
• W2108452826 cites W2172213098 @default.
• W2108452826 cites W2231081458 @default.
• W2108452826 cites W2290856323 @default.
• W2108452826 cites W2413107236 @default.
• W2108452826 cites W4293247451 @default.
• W2108452826 doi "https://doi.org/10.1074/jbc.270.3.1429" @default.
• W2108452826 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7836411" @default.
• W2108452826 hasPublicationYear "1995" @default.
• W2108452826 type Work @default.
• W2108452826 sameAs 2108452826 @default.
• W2108452826 citedByCount "51" @default.
• W2108452826 countsByYear W21084528262013 @default.
• W2108452826 crossrefType "journal-article" @default.
• W2108452826 hasAuthorship W2108452826A5000851275 @default.
• W2108452826 hasAuthorship W2108452826A5001047555 @default.
• W2108452826 hasAuthorship W2108452826A5003978275 @default.
• W2108452826 hasAuthorship W2108452826A5004302932 @default.
• W2108452826 hasAuthorship W2108452826A5035253921 @default.
• W2108452826 hasAuthorship W2108452826A5080858056 @default.
• W2108452826 hasBestOaLocation W21084528261 @default.
• W2108452826 hasConcept C104317684 @default.
• W2108452826 hasConcept C12296475 @default.
• W2108452826 hasConcept C127078168 @default.
• W2108452826 hasConcept C144647389 @default.
• W2108452826 hasConcept C161200384 @default.
• W2108452826 hasConcept C205668238 @default.
• W2108452826 hasConcept C2777485865 @default.
• W2108452826 hasConcept C2779490540 @default.
• W2108452826 hasConcept C41625074 @default.
• W2108452826 hasConcept C55493867 @default.

• next