Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108506513> ?p ?o ?g. }
- W2108506513 endingPage "492" @default.
- W2108506513 startingPage "481" @default.
- W2108506513 abstract "To better understand the transcriptional machinery that governs glucokinase (GCK) expression, we have cloned and characterized the proximal promoter region of GCK from gilthead sea bream ( Sparus aurata ). The 5′-flanking region of GCK was isolated by chromosome walking. SMART RACE-PCR allowed us to locate the transcription start site 98 bp (bp) upstream from the translational start. Transfection analysis in HepG2 cells revealed the presence of a functional promoter in the 1397 bp 5′-flanking isolated fragment (positions −1321 to +76 relative to the transcription start site). Sequential 5′-deletion analysis indicated a core functional promoter for basal transcription within the 288 bp upstream from the transcription start site. Transient transfection experiments performed in HepG2 cells and electrophoretic mobility shift assays denoted that Sp1 binds and transactivates GCK promoter, whereas Sp3 repressed Sp1-mediated activation of GCK by competing for the same binding site. Mutations in the Sp binding site completely abolished the enhancing effect of Sp1. Treatment with insulin stimulated GCK expression, and increased Sp1 levels in S. aurata liver. We propose a new mechanism that involves Sp1 and Sp3 to mediate insulin activation of GCK transcription." @default.
- W2108506513 created "2016-06-24" @default.
- W2108506513 creator A5022514189 @default.
- W2108506513 creator A5071685843 @default.
- W2108506513 creator A5076322154 @default.
- W2108506513 date "2007-04-01" @default.
- W2108506513 modified "2023-10-10" @default.
- W2108506513 title "Sp1 and Sp3 regulate glucokinase gene transcription in the liver of gilthead sea bream (Sparus aurata)" @default.
- W2108506513 cites W1228798347 @default.
- W2108506513 cites W123012959 @default.
- W2108506513 cites W1479842759 @default.
- W2108506513 cites W1520184516 @default.
- W2108506513 cites W1598767975 @default.
- W2108506513 cites W1788294099 @default.
- W2108506513 cites W1964899714 @default.
- W2108506513 cites W1965372133 @default.
- W2108506513 cites W1970434178 @default.
- W2108506513 cites W1972116576 @default.
- W2108506513 cites W1987687515 @default.
- W2108506513 cites W1989915203 @default.
- W2108506513 cites W1991354315 @default.
- W2108506513 cites W2001905532 @default.
- W2108506513 cites W2004181770 @default.
- W2108506513 cites W2014526087 @default.
- W2108506513 cites W2015607170 @default.
- W2108506513 cites W2026529272 @default.
- W2108506513 cites W2029296165 @default.
- W2108506513 cites W2032019900 @default.
- W2108506513 cites W2032766070 @default.
- W2108506513 cites W2037134390 @default.
- W2108506513 cites W2046789266 @default.
- W2108506513 cites W2064116660 @default.
- W2108506513 cites W2073926725 @default.
- W2108506513 cites W2074241920 @default.
- W2108506513 cites W2075399347 @default.
- W2108506513 cites W2081195118 @default.
- W2108506513 cites W2089665780 @default.
- W2108506513 cites W2090224663 @default.
- W2108506513 cites W2094105923 @default.
- W2108506513 cites W2106169455 @default.
- W2108506513 cites W2116655531 @default.
- W2108506513 cites W2130783609 @default.
- W2108506513 cites W2134839833 @default.
- W2108506513 cites W2141835056 @default.
- W2108506513 cites W2149124673 @default.
- W2108506513 cites W2163101309 @default.
- W2108506513 cites W2163463351 @default.
- W2108506513 cites W2166486785 @default.
- W2108506513 cites W2167892995 @default.
- W2108506513 cites W2404506793 @default.
- W2108506513 cites W260692683 @default.
- W2108506513 cites W3145802305 @default.
- W2108506513 doi "https://doi.org/10.1677/jme.1.02176" @default.
- W2108506513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17446237" @default.
- W2108506513 hasPublicationYear "2007" @default.
- W2108506513 type Work @default.
- W2108506513 sameAs 2108506513 @default.
- W2108506513 citedByCount "19" @default.
- W2108506513 countsByYear W21085065132012 @default.
- W2108506513 countsByYear W21085065132013 @default.
- W2108506513 countsByYear W21085065132014 @default.
- W2108506513 countsByYear W21085065132015 @default.
- W2108506513 countsByYear W21085065132017 @default.
- W2108506513 countsByYear W21085065132018 @default.
- W2108506513 countsByYear W21085065132023 @default.
- W2108506513 crossrefType "journal-article" @default.
- W2108506513 hasAuthorship W2108506513A5022514189 @default.
- W2108506513 hasAuthorship W2108506513A5071685843 @default.
- W2108506513 hasAuthorship W2108506513A5076322154 @default.
- W2108506513 hasBestOaLocation W21085065131 @default.
- W2108506513 hasConcept C101762097 @default.
- W2108506513 hasConcept C104317684 @default.
- W2108506513 hasConcept C107824862 @default.
- W2108506513 hasConcept C138885662 @default.
- W2108506513 hasConcept C150194340 @default.
- W2108506513 hasConcept C153911025 @default.
- W2108506513 hasConcept C179926584 @default.
- W2108506513 hasConcept C183978625 @default.
- W2108506513 hasConcept C41895202 @default.
- W2108506513 hasConcept C54009773 @default.
- W2108506513 hasConcept C54355233 @default.
- W2108506513 hasConcept C86339819 @default.
- W2108506513 hasConcept C86803240 @default.
- W2108506513 hasConcept C95444343 @default.
- W2108506513 hasConcept C98335778 @default.
- W2108506513 hasConceptScore W2108506513C101762097 @default.
- W2108506513 hasConceptScore W2108506513C104317684 @default.
- W2108506513 hasConceptScore W2108506513C107824862 @default.
- W2108506513 hasConceptScore W2108506513C138885662 @default.
- W2108506513 hasConceptScore W2108506513C150194340 @default.
- W2108506513 hasConceptScore W2108506513C153911025 @default.
- W2108506513 hasConceptScore W2108506513C179926584 @default.
- W2108506513 hasConceptScore W2108506513C183978625 @default.
- W2108506513 hasConceptScore W2108506513C41895202 @default.
- W2108506513 hasConceptScore W2108506513C54009773 @default.
- W2108506513 hasConceptScore W2108506513C54355233 @default.
- W2108506513 hasConceptScore W2108506513C86339819 @default.
- W2108506513 hasConceptScore W2108506513C86803240 @default.