Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108518355> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2108518355 endingPage "52" @default.
- W2108518355 startingPage "41" @default.
- W2108518355 abstract "The goal of the present study was to identify the molecular mechanism underlying desensitization of endothelin-1 receptor-mediated phosphoinositide response in cultured neonatal rat heart cells. Endothelin elicited a concentration-dependent (EC50=2.2 × 10-9 M) increase of inositolphosphate production with a much higher potency then phenylephrine (EC50=1.4 × 10-6 M). Endothelin-1 (10-8 M) evoked phosphoinositide turnover in the presence of 10 mM LiCl, which was greatly attenuated after 30-45 min of continuous stimulation with agonist, apparently resulting in a total absence of further inositolphosphate accumulation. However, when the uncompetitive inositol monophosphatase inhibitor Li+ was only present during the last 30 min of 150 min incubation, the inositolphosphate accumulation was decreased to a steady state of 33% of the initial rate. The loss of responsiveness of cardiomyocytes to endothelin-1 was not brought about by a limiting supply of phospholipase C substrate phosphatidylinositol 4,5-bishosphate. A very rapid resynthesis of this substrate took place as its level remained almost constant during 45 min stimulation with 10-8M endothelin-1 while the accumulation of inositolphosphates was at least 15-fold higher than the initial cellular phosphatidylinositol 4,5-bisphosphate content. After 120 min preincubation of cells with 10-9M endothelin-1 the activation of phospholipase C by a second higher dose (10-8 M) was severely (67%) inhibited at the same time leaving the induction of phosphoinositide turnover by phenylephrine (10-4 M) virtually intact. Preincubation with phenylephrine (3 × 10-6 M) also led to inhibition of the phenylephrine (10-4 M) mediated inositolphosphate response (36% inhibition) while the endothelin-1 (10-8 M) response was not affected. Addition of a direct activator of protein kinase C, phorbol 12-myristate 13-acetate, led to inhibition of the endothelin-1 evoked phosphoinositide turnover but the rate of desensitization was not affected. Inhibition of protein kinase C with staurosporine did not alter the time course of desensitization. In conclusion, the activity of the phosphoinositide cycle in cardiomyocytes is homologously desensitized after stimulation with endothelin-1. The desensitization is not likely to be due to either depletion of phospholipase C substrate or to the activation of protein kinase C by inositol 1,4,5-trisphosphate-mobilized Ca2+ and elevated 1,2-diacylglycerol levels." @default.
- W2108518355 created "2016-06-24" @default.
- W2108518355 creator A5058971565 @default.
- W2108518355 creator A5065630525 @default.
- W2108518355 creator A5075333641 @default.
- W2108518355 creator A5079920375 @default.
- W2108518355 date "1993-01-01" @default.
- W2108518355 modified "2023-10-15" @default.
- W2108518355 title "Homologous Desensitization of the Endothelin-1 Receptor Mediated Phosphoinositide Response in Cultured Neonatal Rat Cardiomyocytes" @default.
- W2108518355 doi "https://doi.org/10.1006/jmcc.1993.1006" @default.
- W2108518355 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8382749" @default.
- W2108518355 hasPublicationYear "1993" @default.
- W2108518355 type Work @default.
- W2108518355 sameAs 2108518355 @default.
- W2108518355 citedByCount "33" @default.
- W2108518355 countsByYear W21085183552022 @default.
- W2108518355 crossrefType "journal-article" @default.
- W2108518355 hasAuthorship W2108518355A5058971565 @default.
- W2108518355 hasAuthorship W2108518355A5065630525 @default.
- W2108518355 hasAuthorship W2108518355A5075333641 @default.
- W2108518355 hasAuthorship W2108518355A5079920375 @default.
- W2108518355 hasConcept C126322002 @default.
- W2108518355 hasConcept C134018914 @default.
- W2108518355 hasConcept C144980905 @default.
- W2108518355 hasConcept C149601957 @default.
- W2108518355 hasConcept C170493617 @default.
- W2108518355 hasConcept C185592680 @default.
- W2108518355 hasConcept C24998067 @default.
- W2108518355 hasConcept C2777952589 @default.
- W2108518355 hasConcept C2778597717 @default.
- W2108518355 hasConcept C2778938600 @default.
- W2108518355 hasConcept C2780610907 @default.
- W2108518355 hasConcept C55493867 @default.
- W2108518355 hasConcept C62478195 @default.
- W2108518355 hasConcept C71924100 @default.
- W2108518355 hasConcept C84393581 @default.
- W2108518355 hasConcept C86803240 @default.
- W2108518355 hasConcept C92759361 @default.
- W2108518355 hasConceptScore W2108518355C126322002 @default.
- W2108518355 hasConceptScore W2108518355C134018914 @default.
- W2108518355 hasConceptScore W2108518355C144980905 @default.
- W2108518355 hasConceptScore W2108518355C149601957 @default.
- W2108518355 hasConceptScore W2108518355C170493617 @default.
- W2108518355 hasConceptScore W2108518355C185592680 @default.
- W2108518355 hasConceptScore W2108518355C24998067 @default.
- W2108518355 hasConceptScore W2108518355C2777952589 @default.
- W2108518355 hasConceptScore W2108518355C2778597717 @default.
- W2108518355 hasConceptScore W2108518355C2778938600 @default.
- W2108518355 hasConceptScore W2108518355C2780610907 @default.
- W2108518355 hasConceptScore W2108518355C55493867 @default.
- W2108518355 hasConceptScore W2108518355C62478195 @default.
- W2108518355 hasConceptScore W2108518355C71924100 @default.
- W2108518355 hasConceptScore W2108518355C84393581 @default.
- W2108518355 hasConceptScore W2108518355C86803240 @default.
- W2108518355 hasConceptScore W2108518355C92759361 @default.
- W2108518355 hasIssue "1" @default.
- W2108518355 hasLocation W21085183551 @default.
- W2108518355 hasLocation W21085183552 @default.
- W2108518355 hasOpenAccess W2108518355 @default.
- W2108518355 hasPrimaryLocation W21085183551 @default.
- W2108518355 hasRelatedWork W1585012800 @default.
- W2108518355 hasRelatedWork W1970652449 @default.
- W2108518355 hasRelatedWork W2016066140 @default.
- W2108518355 hasRelatedWork W2027294752 @default.
- W2108518355 hasRelatedWork W2040399427 @default.
- W2108518355 hasRelatedWork W2040974472 @default.
- W2108518355 hasRelatedWork W2081610379 @default.
- W2108518355 hasRelatedWork W2094178034 @default.
- W2108518355 hasRelatedWork W2236098356 @default.
- W2108518355 hasRelatedWork W3084225630 @default.
- W2108518355 hasVolume "25" @default.
- W2108518355 isParatext "false" @default.
- W2108518355 isRetracted "false" @default.
- W2108518355 magId "2108518355" @default.
- W2108518355 workType "article" @default.