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- W2108524012 abstract "The sequential enzyme assay as previously described has been used to study various effects on the three enzymes in human red cells involved in the phosphorylation of galactose: galactokinase, galactose-1-phosphate uridyl transferase and uridine diphospho-galactose-4-epimerase. 1 Enzyme activities in undiluted lysates appear to reflect the respective activities in whole cells. 2 Added extracellular Gal-1-P, G-1-P, UDPGal and UPDG do not affect enzyme activities in whole cells. 3 The kinase and transferase enzymes do not appear to be associated with the membrane fraction of the red cells. 4 Galactokinase activity is inhibited by G-6-P and Gal-1-P, but not by glucose, G-1-P, UDPG, UDPGal, UTP or NAD+. It is inhibited by ATP and ADP in high concentration. 5 Galactose-1-phosphate uridyl transferase activity is inhibited by G-1-P, G-6-P, UDPG, UDPGal, ATP, and ADP. It is not affected by UTP, NAD+, or galactose. 6 Uridine diphospho-galactose-4-epimerase activity is inhibited by UDPG, ATP, ADP, UTP and NADH. It is stimulated by NAD+ and possibly by Gal-1-P. It is unaffected by G-1-P, G-6-P. 7 The rates of the three reactions decrease with decreasing temperature. The activities of transferase and epimerase are inactivated at the same rate, the kinase activity is inactivated more slowly. 8 Dilution experiments indicate the presence in lysates of a pool of UDPG (or, possibly UDPGal) which regulates the activities transferase and the epimerase enzymes. 9 Results of dilution experiments suggest that the radioactive product of the transferase enzyme is different from commercially available UDPGal-u-14C. 10 ATP, UTP and UDPG interact with some substance(s) in the red cell lysate to cause a time dependent inactivation of the epimerase. These interactions are the result of glucose metabolism." @default.
- W2108524012 created "2016-06-24" @default.
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- W2108524012 date "1971-12-01" @default.
- W2108524012 modified "2023-09-24" @default.
- W2108524012 title "Enzyme kinetics in mammalian cells. III. Regulation of activities of galactokinase, galactose-1-phosphate uridyl transferase and uridine diphosphogalactose-4-epimerase in human erythrocytes" @default.
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- W2108524012 doi "https://doi.org/10.1002/jcp.1040780311" @default.
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