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- W2108559901 abstract "Background. During peritoneal dialysis (PD), mesothelial cells (MC) undergo an epithelial-to-mesenchymal transition (EMT), and this process is associated with peritoneal membrane (PM) damage. Bone morphogenic protein-7 (BMP-7) antagonizes transforming growth factor (TGF)-β1, modulates EMT and protects against fibrosis. Herein, we analysed the modulating role of BMP-7 on EMT of MC in vitro and its protective effects in a rat PD model. Methods. Epitheliod or non-epitheliod MC were analysed for the expression of BMP-7, TGF-β1, activated Smads, epithelial cadherin (E-cadherin), collagen I, alpha smooth muscle cell actin (α-SMA) and vascular endothelial growth factor (VEGF) using standard procedures. Rats were daily instilled with PD fluid with or without BMP-7 during 5 weeks. Histological analyses were carried out in parietal peritoneum. Fibrosis was quantified with van Gieson or Masson's trichrome staining. Vasculature, activated macrophages and invading MC were quantified by immunofluorescence analysis. Quantification of infiltrating leukocytes and MC density in liver imprints was performed by May–Grünwald–Giemsa staining. Hyaluronic acid levels were determined by ELISA. Results. MC constitutively expressed BMP-7, and its expression was downregulated during EMT. Treatment with recombinant BMP-7 resulted in blockade of TGF-β1-induced EMT of MC. We provide evidence of a Smad-dependent mechanism for the blockade of EMT. Exposure of rat peritoneum to PD fluid resulted in inflammatory and regenerative responses, invasion of the compact zone by MC, fibrosis and angiogenesis. Administration of BMP-7 decreased the number of invading MC and reduced fibrosis and angiogenesis. In contrast, BMP-7 had no effect on inflammatory and regenerative responses, suggesting that these are EMT-independent, and probably upstream, processes. Conclusions. Data point to a balance between BMP-7 and TGF-β1 in the control of EMT and indicate that blockade of EMT may be a therapeutic approach to ameliorate peritoneal membrane damage during PD." @default.
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- W2108559901 date "2010-01-12" @default.
- W2108559901 modified "2023-10-18" @default.
- W2108559901 title "BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposure" @default.
- W2108559901 cites W1636022999 @default.
- W2108559901 cites W1743986849 @default.
- W2108559901 cites W1971318940 @default.
- W2108559901 cites W1982125065 @default.
- W2108559901 cites W1986164061 @default.
- W2108559901 cites W1986267718 @default.
- W2108559901 cites W1993345123 @default.
- W2108559901 cites W1994738588 @default.
- W2108559901 cites W1998000347 @default.
- W2108559901 cites W2015740961 @default.
- W2108559901 cites W2017215688 @default.
- W2108559901 cites W2035175400 @default.
- W2108559901 cites W2036755333 @default.
- W2108559901 cites W2053302971 @default.
- W2108559901 cites W2059122016 @default.
- W2108559901 cites W2060245754 @default.
- W2108559901 cites W2080431549 @default.
- W2108559901 cites W2082429467 @default.
- W2108559901 cites W2087757614 @default.
- W2108559901 cites W2088876544 @default.
- W2108559901 cites W2093157637 @default.
- W2108559901 cites W2097603228 @default.
- W2108559901 cites W2100891344 @default.
- W2108559901 cites W2101417810 @default.
- W2108559901 cites W2103321505 @default.
- W2108559901 cites W2105621799 @default.
- W2108559901 cites W2106141385 @default.
- W2108559901 cites W2109974825 @default.
- W2108559901 cites W2117324021 @default.
- W2108559901 cites W2117425971 @default.
- W2108559901 cites W2118254829 @default.
- W2108559901 cites W2121831945 @default.
- W2108559901 cites W2130322678 @default.
- W2108559901 cites W2131251311 @default.
- W2108559901 cites W2143992450 @default.
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- W2108559901 cites W2148184356 @default.
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- W2108559901 cites W2160293072 @default.
- W2108559901 cites W2161918376 @default.
- W2108559901 cites W2168456732 @default.
- W2108559901 cites W2171452911 @default.
- W2108559901 cites W2185902145 @default.
- W2108559901 cites W2267776909 @default.
- W2108559901 cites W2335187016 @default.
- W2108559901 cites W2339783540 @default.
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- W2108559901 doi "https://doi.org/10.1093/ndt/gfp618" @default.
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