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- W2108587248 abstract "Summary In uropathogenic Escherichia coli strain 536, six pathogenicity islands (PAIs) encode key virulence factors. All PAIs except PAI IV 536 are flanked by direct repeats and four of them encode integrases responsible for their chromosomal excision. To study recombination sites used for the integration by PAI II 536 and III 536 integrases, we measured site‐specific recombination between the chromosomal integration site attB , and the PAI‐specific attachment site attP . We show that PAI III 536 IntB, but not IntA, mediates PAI III 536 integration. Studies of integrative recombination sites of both PAIs show that, when using a large cognate attP site (839 bp for PAI II 536 and 268 bp for PAI III 536 ), PAI II 536 and III 536 attB sites could be reduced to 16 bp and 20 bp, respectively, without affecting recombination. Further reduction to 14 bp for PAI II 536 and 13 bp for PAI III 536 diminished recombination efficiency. Surprisingly, attP sites could also be reduced to 14 bp (PAI II 536 ) and 20 bp (PAI III 536 ). The integration host factor (IHF) and the DNA‐bending HU protein do not influence PAI II 536 recombination, but IHF enhances PAI‐III 536 excision and negatively affects its integration. These data suggest that PAI intasomes differ from those of lambda and P4 integrase paradigms." @default.
- W2108587248 created "2016-06-24" @default.
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- W2108587248 date "2008-02-26" @default.
- W2108587248 modified "2023-10-02" @default.
- W2108587248 title "Delineation of the recombination sites necessary for integration of pathogenicity islands II and III into the Escherichia coli 536 chromosome" @default.
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- W2108587248 doi "https://doi.org/10.1111/j.1365-2958.2008.06145.x" @default.
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