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• W2108630453 abstract "In this study, laboratory-reared houseflies were experimentally exposed to the high pathogenicity avian influenza virus (HPAI) subtype H5N1 virus to evaluate the houseflies as vectors in HPAI-H5N1 virus transmission in chickens. One hundred and fifty houseflies (Musca domestica L.) were equally allocated into three groups. Groups 2 and 3 were exposed to the HPAI-H5N1 virus by allowing the flies to consume food containing the virus for 15 min, while the flies in group 1 were allowed to consume H5N1-free food and would serve as a negative control group. Group 2 flies were euthanatized immediately after H5N1 exposure, while group 3 were held at room temperature for 24 hr and euthanatized. The houseflies in the transmission of the HPAI-H5N1 virus were examined by challenging three groups of housefly homogenates into layer chickens via the oral drop. Morbidity and mortality were observed for 14 days, and virus shedding monitored via oropharyngeal swabs (OS) and cloacal swabs (CS), which were collected daily and determined by real-time reverse transcription-PCR and virus titration. Experimental challenge showed that all the chickens of groups 2 and 3 died within 7 days of inoculation. The OS had higher concentrations of virus than CS. Moreover, the chickens of group 2 had higher concentrations of virus shedding than the chickens of group 3. Immunohistochemistry detected the nucleoprotein of the type A influenza virus in all tissue samples collected, including the trachea, duodenum, pancreas, and brain. In summary, this study demonstrates that houseflies could serve as vectors in HPAI-H5N1 virus transmission in chickens under experimental conditions.Evaluación experimental de las moscas domésticas como vectores para la transmisión a los pollos del virus de la influenza aviar subtipo H5N1.En este estudio, moscas domésticas criadas en laboratorio se expusieron experimentalmente al virus de la influenza aviar de alta patogenicidad subtipo H5N1 para evaluar a estos insectos como vectores para la transmisión de este virus a los pollos. Ciento cincuenta moscas domésticas (Musca domestica L.) se asignaron de manera igual en tres grupos. Los grupos dos y tres fueron expuestos al virus de la influenza aviar alimentándolas por 15 minutos con alimento que contenía el virus, mientras que a las moscas en el grupo uno se les permitió consimir alimento libre del virus y servir como control negativo. A las moscas del grupo dos se les practicó la eutanasia inmediatamente después de la exposición con el virus de influenza aviar H5N, mientras que el grupo tres se mantuvo a temperatura ambiente por 24 horas antes de ser sometidas a la eutanasia. Las moscas domésticas se examinaron en la transmisión del virus de influenza aviar de alta patogenicidad H5N1 mediante el desafío de aves de postura con macerados de moscas por vía oral. Se observaron la morbilidad y la mortalidad por 14 días, y la eliminación viral se determinó mediante hisopos orofaríngeos y cloacales, los cuales fueron recolectados diariamente se analizaron por transcripción reversa y PCR en tiempo real además por titulación viral. El desafío experimental mostró que todos los pollos de los grupos dos y tres murieron dentro de los siete días después de la inoculación. Los hisopos orofaríngeos mostraron las concentraciones virales más altas en comparación con los hisopos cloacales. Además, los pollos del grupo 2 mostraron concentraciones más altas de eliminación viral en comparación con el grupo tres. Mediante inmunohistoquímica, se detectó a la nucleoproteína del virus de la influenza A en todas muestras de tejidos recolectadas, incluyendo la tráquea duodeno, páncreas y cerebro. En resumen, este estudio demuestra que las moscas domésticas pueden servir como vectores en la transmisión del virus de la influenza aviar de alta patogenicidad subtipo H5N1 a los pollos bajo condiciones experimentales." @default.
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• W2108630453 date "2013-06-01" @default.
• W2108630453 modified "2023-10-14" @default.
• W2108630453 title "Experimental Assessment of Houseflies as Vectors in Avian Influenza Subtype H5N1 Transmission in Chickens" @default.
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• W2108630453 doi "https://doi.org/10.1637/10347-090412-reg.1" @default.
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