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• W2108654404 abstract "No AccessJournal of UrologyInvestigative urology1 Jan 2006Novel Urothelium Specific Gene Expression Identified by Differential Display Reverse Transcriptase-Polymerase Chain Reactionis accompanied byHow Far are We From a Urothelial Gene Chip? G.D. Hall, B. Smith, R.J. Weeks, P.J. Selby, J. Southgate, and J.D. Chester G.D. HallG.D. Hall More articles by this author , B. SmithB. Smith Current address: Jack Birch Unit for Molecular Carcinogenesis, Department of Biology, University of York, YO10 5YW, United Kingdom. More articles by this author , R.J. WeeksR.J. Weeks Current address: Cancer Genetics Laboratory, Department of Biochemistry, University of Otago, Dunedin, New Zealand. More articles by this author , P.J. SelbyP.J. Selby More articles by this author , J. SouthgateJ. Southgate Current address: Jack Birch Unit for Molecular Carcinogenesis, Department of Biology, University of York, YO10 5YW, United Kingdom. Equal study contribution. More articles by this author , and J.D. ChesterJ.D. Chester Equal study contribution. More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)00006-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Understanding the molecular basis of differential gene expression among different tissues at various developmental stages and in neoplastic transformation is an important biological goal. The potential clinical applications of this improved understanding are more precise diagnosis of disease, prediction of prognosis, novel targeted therapies and prediction of response to therapy. Materials and Methods: Differential display reverse transcriptase-polymerase chain reaction was used to compare gene expression in bovine urothelium to that in autologous lung, esophagus, liver and spleen. Products that appeared to have urothelial specific expression were sequenced and assessed for homology with known sequences. Ribonuclease protection assays were used to further confirm the expression pattern. Results: A total of 32 discrete cDNAs were identified, including 3 products from genes known to be urothelium specific in their expression, 16 with significant homology to bovine, human or mouse expressed sequence tags and 5 with no sequence homology to any currently available sequence. Urothelium specific mRNA expression was confirmed for 3 genes by ribonuclease protection assays and one (Udd06) was further characterized as a urea transporter. Conclusions: The use of differential display reverse transcriptase-polymerase chain reaction and other complementary techniques for parallel gene expression analysis will permit the complete characterization of the urothelial transcriptome and help identify potential molecular targets for rationally targeted therapy. References 1 : Uroplakins as markers of urothelial differentiation. Adv Exp Med Biol1999; 462: 7. Google Scholar 2 : Uroplakin gene expression by normal and neoplastic human urothelium. Am J Pathol1998; 153: 1957. Google Scholar 3 : Uroplakin gene expression in normal human tissues and locally advanced bladder cancer. J Pathol2003; 199: 41. Google Scholar 4 : Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science1995; 270: 467. Google Scholar 5 : Serial analysis of gene expression. Science1995; 270: 484. Google Scholar 6 : Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science1992; 257: 967. Google Scholar 7 : Differential display cloning identifies motility-related protein (MRP1/CD9) as highly expressed in primary compared to metastatic human colon carcinoma cells. Cancer Res1997; 57: 2593. Google Scholar 8 : Identification of genes differentially expressed in testes containing carcinoma in situ. Mol Hum Reprod2004; 10: 423. Google Scholar 9 : Gene expression analysis of tumor spheroids reveals a role for suppressed DNA mismatch repair in multicellular resistance to alkylating agents. Mol Cell Biol2004; 24: 6837. Google Scholar 10 : Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle. Genome Res2001; 11: 626. Google Scholar 11 : Normal human urothelial cells in vitro: proliferation and induction of stratification. Lab Invest1994; 71: 583. Google Scholar 12 : Identification of genes involved in human urothelial cell-matrix interactions: implications for the progression pathways of malignant urothelium. Cancer Res2001; 61: 1678. Google Scholar 13 National Centre for Biotechnology Information, 2005. Available at http://www.ncbi.nlm.nih.gov/blast/. Accessed March 2005 Google Scholar 14 : Mammalian urea transporters. Annu Rev Physiol2003; 65: 543. Google Scholar 15 : Structure, regulation and physiological roles of urea transporters. Kidney Int1996; 49: 1615. Google Scholar 16 : Regulation of urea permeability in frog urinary bladder by prostaglandin E(2). Pflugers Arch2002; 444: 159. Google Scholar Cancer Research UK Clinical Centre in Leeds, St James’s University Hospital, Leeds, United Kingdom© 2006 by American Urological AssociationFiguresReferencesRelatedDetailsCited byAtala A (2018) Re: Expression and Localization of a UT-B Urea Transporter in the Human BladderJournal of Urology, VOL. 194, NO. 2, (592-594), Online publication date: 1-Aug-2015.Related articlesJournal of Urology9 Nov 2018How Far are We From a Urothelial Gene Chip? Volume 175Issue 1January 2006Page: 337-342 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordsgene therapyurotheliumbladderpolymerase chain reactionurea transporterMetricsAuthor Information G.D. Hall More articles by this author B. Smith Current address: Jack Birch Unit for Molecular Carcinogenesis, Department of Biology, University of York, YO10 5YW, United Kingdom. More articles by this author R.J. Weeks Current address: Cancer Genetics Laboratory, Department of Biochemistry, University of Otago, Dunedin, New Zealand. More articles by this author P.J. Selby More articles by this author J. Southgate Current address: Jack Birch Unit for Molecular Carcinogenesis, Department of Biology, University of York, YO10 5YW, United Kingdom. Equal study contribution. More articles by this author J.D. Chester Equal study contribution. More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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