FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108658220> ?p ?o ?g. }

• W2108658220 abstract "Abstract Background Safflower (Carthamus tinctorius L.) is a difficult crop to genetically transform being susceptible to hyperhydration and poor in vitro root formation. In addition to traditional uses safflower has recently emerged as a broadacre platform for the production of transgenic products including modified oils and pharmaceutically active proteins. Despite commercial activities based on the genetic modification of safflower, there is no method available in the public domain describing the transformation of safflower that generates transformed T 1 progeny. Results An efficient and reproducible protocol has been developed with a transformation efficiency of 4.8% and 3.1% for S-317 (high oleic acid content) and WT (high linoleic acid content) genotypes respectively. An improved safflower transformation T-DNA vector was developed, including a secreted GFP to allow non-destructive assessment of transgenic shoots. Hyperhydration and necrosis of Agrobacterium -infected cotyledons was effectively controlled by using iota-carrageenan, L-cysteine and ascorbic acid. To overcome poor in vitro root formation for the first time a grafting method was developed for safflower in which ~50% of transgenic shoots develop into mature plants bearing viable transgenic T 1 seed. The integration and expression of secreted GFP and hygromycin genes were confirmed by PCR, Southern and Western blot analysis. Southern blot analysis in nine independent lines indicated that 1-7 transgenes were inserted per line and T 1 progeny displayed Mendelian inheritance. Conclusions This protocol demonstrates significant improvements in both the efficiency and ease of use over existing safflower transformation protocols. This is the first complete method of genetic transformation of safflower that generates stably-transformed plants and progeny, allowing this crop to benefit from modern molecular applications." @default.
• W2108658220 created "2016-06-24" @default.
• W2108658220 creator A5009861802 @default.
• W2108658220 creator A5027699834 @default.
• W2108658220 creator A5037187950 @default.
• W2108658220 creator A5041635820 @default.
• W2108658220 creator A5055102597 @default.
• W2108658220 date "2011-05-20" @default.
• W2108658220 modified "2023-10-17" @default.
• W2108658220 title "Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting" @default.
• W2108658220 cites W1264646771 @default.
• W2108658220 cites W173059665 @default.
• W2108658220 cites W1764587948 @default.
• W2108658220 cites W1841521301 @default.
• W2108658220 cites W1963566729 @default.
• W2108658220 cites W1967092449 @default.
• W2108658220 cites W1990309253 @default.
• W2108658220 cites W1992457727 @default.
• W2108658220 cites W1994717043 @default.
• W2108658220 cites W1996605201 @default.
• W2108658220 cites W2003169159 @default.
• W2108658220 cites W2024556021 @default.
• W2108658220 cites W2025078829 @default.
• W2108658220 cites W2051598894 @default.
• W2108658220 cites W2056279449 @default.
• W2108658220 cites W2058039066 @default.
• W2108658220 cites W2077105570 @default.
• W2108658220 cites W2085510068 @default.
• W2108658220 cites W2094213965 @default.
• W2108658220 cites W2136796097 @default.
• W2108658220 cites W2138446061 @default.
• W2108658220 cites W2143651768 @default.
• W2108658220 cites W2145078836 @default.
• W2108658220 cites W2316537020 @default.
• W2108658220 doi "https://doi.org/10.1186/1746-4811-7-12" @default.
• W2108658220 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3115923" @default.
• W2108658220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21595986" @default.
• W2108658220 hasPublicationYear "2011" @default.
• W2108658220 type Work @default.
• W2108658220 sameAs 2108658220 @default.
• W2108658220 citedByCount "47" @default.
• W2108658220 countsByYear W21086582202012 @default.
• W2108658220 countsByYear W21086582202013 @default.
• W2108658220 countsByYear W21086582202014 @default.
• W2108658220 countsByYear W21086582202015 @default.
• W2108658220 countsByYear W21086582202016 @default.
• W2108658220 countsByYear W21086582202017 @default.
• W2108658220 countsByYear W21086582202018 @default.
• W2108658220 countsByYear W21086582202019 @default.
• W2108658220 countsByYear W21086582202020 @default.
• W2108658220 countsByYear W21086582202021 @default.
• W2108658220 countsByYear W21086582202022 @default.
• W2108658220 countsByYear W21086582202023 @default.
• W2108658220 crossrefType "journal-article" @default.
• W2108658220 hasAuthorship W2108658220A5009861802 @default.
• W2108658220 hasAuthorship W2108658220A5027699834 @default.
• W2108658220 hasAuthorship W2108658220A5037187950 @default.
• W2108658220 hasAuthorship W2108658220A5041635820 @default.
• W2108658220 hasAuthorship W2108658220A5055102597 @default.
• W2108658220 hasBestOaLocation W21086582201 @default.
• W2108658220 hasConcept C102230213 @default.
• W2108658220 hasConcept C104317684 @default.
• W2108658220 hasConcept C122172344 @default.
• W2108658220 hasConcept C144027150 @default.
• W2108658220 hasConcept C178050479 @default.
• W2108658220 hasConcept C204241405 @default.
• W2108658220 hasConcept C2779656422 @default.
• W2108658220 hasConcept C2781451173 @default.
• W2108658220 hasConcept C2986274086 @default.
• W2108658220 hasConcept C54355233 @default.
• W2108658220 hasConcept C556039675 @default.
• W2108658220 hasConcept C71924100 @default.
• W2108658220 hasConcept C86803240 @default.
• W2108658220 hasConceptScore W2108658220C102230213 @default.
• W2108658220 hasConceptScore W2108658220C104317684 @default.
• W2108658220 hasConceptScore W2108658220C122172344 @default.
• W2108658220 hasConceptScore W2108658220C144027150 @default.
• W2108658220 hasConceptScore W2108658220C178050479 @default.
• W2108658220 hasConceptScore W2108658220C204241405 @default.
• W2108658220 hasConceptScore W2108658220C2779656422 @default.
• W2108658220 hasConceptScore W2108658220C2781451173 @default.
• W2108658220 hasConceptScore W2108658220C2986274086 @default.
• W2108658220 hasConceptScore W2108658220C54355233 @default.
• W2108658220 hasConceptScore W2108658220C556039675 @default.
• W2108658220 hasConceptScore W2108658220C71924100 @default.
• W2108658220 hasConceptScore W2108658220C86803240 @default.
• W2108658220 hasIssue "1" @default.
• W2108658220 hasLocation W21086582201 @default.
• W2108658220 hasLocation W21086582202 @default.
• W2108658220 hasLocation W21086582203 @default.
• W2108658220 hasLocation W21086582204 @default.
• W2108658220 hasOpenAccess W2108658220 @default.
• W2108658220 hasPrimaryLocation W21086582201 @default.
• W2108658220 hasRelatedWork W1975403121 @default.
• W2108658220 hasRelatedWork W2065530341 @default.
• W2108658220 hasRelatedWork W2097436584 @default.
• W2108658220 hasRelatedWork W2098647701 @default.
• W2108658220 hasRelatedWork W2108658220 @default.
• W2108658220 hasRelatedWork W2349573545 @default.
• W2108658220 hasRelatedWork W235110655 @default.

• next