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- W2108754500 abstract "Cost-effective, high-performance diagnostic instruments are vital to providing the society with accessible, affordable, and high-quality healthcare. Here we present an integrated, “microfluidic drifting” based flow cytometry chip as a potential inexpensive, fast, and reliable diagnostic tool. It is capable of analyzing human blood for cell counting and diagnosis of diseases. Our device achieves a throughput of ~3754 events/s. Calibration with Flow-Check calibration beads indicated good congruency with a commercially available benchtop flow cytometer. Moreover, subjection to a stringent 8-peak rainbow calibration particle test demonstrated its ability to perform high-resolution immunological studies with separation resolution of 4.28 between the two dimmest fluorescent populations. Counting accuracy at different polystyrene bead concentrations showed strong correlation (r = 0.9991) with hemocytometer results. Finally, reliable quantification of CD4+ cells in healthy human blood via staining with monoclonal antibodies was demonstrated. These results demonstrate the potential of our microfluidic flow cytometry chip as an inexpensive yet high-performance point-of-care device for mobile medicine." @default.
- W2108754500 created "2016-06-24" @default.
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- W2108754500 date "2014-06-04" @default.
- W2108754500 modified "2023-10-17" @default.
- W2108754500 title "Immunological Analyses of Whole Blood via “Microfluidic Drifting” Based Flow Cytometric Chip" @default.
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- W2108754500 doi "https://doi.org/10.1007/s10439-014-1041-5" @default.
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