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• W2108790008 abstract "To compare the chondrogenic and proliferative effects of pooled human serum (HS) and fetal bovine serum (FBS) on tissue-engineered human nasal septal chondrocytes.Human chondrocytes were expanded for one passage in monolayer in medium supplemented with 10% FBS, 2% HS, 10% HS, or 20% HS. Cells were then suspended in alginate beads for 3D culture for 2 weeks with 10% FBS, 2% HS, 10% HS, or 20% HS.Monolayer cell yields were greater with HS than FBS. In alginate, cellular proliferation, glycosaminoglycan production per cell, and type II collagen were significantly higher with 10% HS compared to 10% FBS controls.HS results in increased proliferation and production of cartilaginous extracellular matrix by tissue-engineered human nasal septal chondrocytes, compared to FBS controls.Culture with human serum may facilitate creation of neocartilage constructs that more closely resemble native tissue." @default.
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• W2108790008 date "2006-09-01" @default.
• W2108790008 modified "2023-09-27" @default.
• W2108790008 title "Human serum for tissue engineering of human nasal septal cartilage" @default.
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• W2108790008 doi "https://doi.org/10.1016/j.otohns.2006.05.029" @default.
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