FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108795427> ?p ?o ?g. }

• W2108795427 endingPage "173" @default.
• W2108795427 startingPage "163" @default.
• W2108795427 abstract "The increasing need to multiplex nucleic acid reactions presses test designers to the limits of amplification specificity in PCR. Although more than a dozen hot starts have been developed for PCR to reduce primer-dimer formation, none can stop the propagation of primer-dimers once formed. Even a small number of primer-dimers can result in false-negatives and/or false-positives. Herein, we demonstrate a new class of primer technology that greatly reduces primer-dimer propagation, showing successful amplification of 60 template copies with no signal dampening in a background of 150,000,000 primer-dimers. In contrast, normal primers, with or without a hot start, experienced signal dampening with as few as 60 primer-dimers and false-negatives with only 600 primer-dimers. This represents more than a 2.5 million-fold improvement in reduction of nonspecific amplification. We also show how a probe can be incorporated into the cooperative primer, with 2.5 times more signal than conventional fluorescent probes." @default.
• W2108795427 created "2016-06-24" @default.
• W2108795427 creator A5039938601 @default.
• W2108795427 date "2014-03-01" @default.
• W2108795427 modified "2023-09-30" @default.
• W2108795427 title "Cooperative Primers" @default.
• W2108795427 cites W1559760142 @default.
• W2108795427 cites W169834448 @default.
• W2108795427 cites W1976082549 @default.
• W2108795427 cites W1986534666 @default.
• W2108795427 cites W2001278481 @default.
• W2108795427 cites W2015961030 @default.
• W2108795427 cites W2017829077 @default.
• W2108795427 cites W2033371591 @default.
• W2108795427 cites W2038895087 @default.
• W2108795427 cites W2039146500 @default.
• W2108795427 cites W2060660006 @default.
• W2108795427 cites W2062253025 @default.
• W2108795427 cites W2064063117 @default.
• W2108795427 cites W2069468367 @default.
• W2108795427 cites W2076558135 @default.
• W2108795427 cites W2082588492 @default.
• W2108795427 cites W2087214907 @default.
• W2108795427 cites W2087700805 @default.
• W2108795427 cites W2104279152 @default.
• W2108795427 cites W2120773494 @default.
• W2108795427 cites W2137167287 @default.
• W2108795427 cites W2143632878 @default.
• W2108795427 cites W2148312059 @default.
• W2108795427 cites W4246376691 @default.
• W2108795427 doi "https://doi.org/10.1016/j.jmoldx.2013.10.004" @default.
• W2108795427 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24370857" @default.
• W2108795427 hasPublicationYear "2014" @default.
• W2108795427 type Work @default.
• W2108795427 sameAs 2108795427 @default.
• W2108795427 citedByCount "20" @default.
• W2108795427 countsByYear W21087954272016 @default.
• W2108795427 countsByYear W21087954272017 @default.
• W2108795427 countsByYear W21087954272018 @default.
• W2108795427 countsByYear W21087954272019 @default.
• W2108795427 countsByYear W21087954272020 @default.
• W2108795427 countsByYear W21087954272021 @default.
• W2108795427 countsByYear W21087954272023 @default.
• W2108795427 crossrefType "journal-article" @default.
• W2108795427 hasAuthorship W2108795427A5039938601 @default.
• W2108795427 hasBestOaLocation W21087954271 @default.
• W2108795427 hasConcept C104317684 @default.
• W2108795427 hasConcept C153911025 @default.
• W2108795427 hasConcept C154945302 @default.
• W2108795427 hasConcept C156719811 @default.
• W2108795427 hasConcept C178790620 @default.
• W2108795427 hasConcept C185592680 @default.
• W2108795427 hasConcept C24107716 @default.
• W2108795427 hasConcept C2776294106 @default.
• W2108795427 hasConcept C2777563447 @default.
• W2108795427 hasConcept C2781188995 @default.
• W2108795427 hasConcept C2910367586 @default.
• W2108795427 hasConcept C41008148 @default.
• W2108795427 hasConcept C49105822 @default.
• W2108795427 hasConcept C52001828 @default.
• W2108795427 hasConcept C54355233 @default.
• W2108795427 hasConcept C64869954 @default.
• W2108795427 hasConcept C70721500 @default.
• W2108795427 hasConcept C86803240 @default.
• W2108795427 hasConcept C90583042 @default.
• W2108795427 hasConceptScore W2108795427C104317684 @default.
• W2108795427 hasConceptScore W2108795427C153911025 @default.
• W2108795427 hasConceptScore W2108795427C154945302 @default.
• W2108795427 hasConceptScore W2108795427C156719811 @default.
• W2108795427 hasConceptScore W2108795427C178790620 @default.
• W2108795427 hasConceptScore W2108795427C185592680 @default.
• W2108795427 hasConceptScore W2108795427C24107716 @default.
• W2108795427 hasConceptScore W2108795427C2776294106 @default.
• W2108795427 hasConceptScore W2108795427C2777563447 @default.
• W2108795427 hasConceptScore W2108795427C2781188995 @default.
• W2108795427 hasConceptScore W2108795427C2910367586 @default.
• W2108795427 hasConceptScore W2108795427C41008148 @default.
• W2108795427 hasConceptScore W2108795427C49105822 @default.
• W2108795427 hasConceptScore W2108795427C52001828 @default.
• W2108795427 hasConceptScore W2108795427C54355233 @default.
• W2108795427 hasConceptScore W2108795427C64869954 @default.
• W2108795427 hasConceptScore W2108795427C70721500 @default.
• W2108795427 hasConceptScore W2108795427C86803240 @default.
• W2108795427 hasConceptScore W2108795427C90583042 @default.
• W2108795427 hasIssue "2" @default.
• W2108795427 hasLocation W21087954271 @default.
• W2108795427 hasLocation W21087954272 @default.
• W2108795427 hasOpenAccess W2108795427 @default.
• W2108795427 hasPrimaryLocation W21087954271 @default.
• W2108795427 hasRelatedWork W130005281 @default.
• W2108795427 hasRelatedWork W169075148 @default.
• W2108795427 hasRelatedWork W1971029043 @default.
• W2108795427 hasRelatedWork W2080534576 @default.
• W2108795427 hasRelatedWork W2094290210 @default.
• W2108795427 hasRelatedWork W2108795427 @default.
• W2108795427 hasRelatedWork W2320641230 @default.
• W2108795427 hasRelatedWork W2403090579 @default.
• W2108795427 hasRelatedWork W3084381115 @default.

• next