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• W2108797777 abstract "The structural characterization of unknown oligosaccharides remains a big challenge since a large number of isomeric structures are possible even for disaccharides. In this work, electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) was used for the differentiation of isomeric pentose disaccharides, α-(1 → 5)-L-arabinobiose (Ara(2)) and β-(1 → 4)-D-xylobiose (Xyl(2)).ESI-MS/MS spectra of [M + Li](+) and [M + Na](+) ions of Ara(2) and Xyl(2), as well as these precursor ions of (18)O-labelled disaccharides, were acquired using two mass spectrometers equipped with different analyzers: LIT (linear ion trap) and Q-TOF (quadrupole time-of-flight).Product ions observed in MS/MS spectra arise from the cleavage at the nonreducing side of the glycosidic bond (Y(1)(+)) and from cross-ring cleavages (0,1)A(2)(+), (0,2)A(2)(+), and (0,3)A(2)(+) at the reducing residue. Statistically significant differences were observed between the relative abundance of specific product ions, when comparing both disaccharides. These differences allowed discriminant models to be built and to propose a criterion using the relative abundances of selected ions capable of discriminating between the isomers for both adduct ions and spectrometers.Isomeric pentose disaccharides can be distinguished based on the fragmentation of both [M + Li](+) and [M + Na](+) ions and using different mass spectrometers. However, LIT instrument has a better discriminant power." @default.
• W2108797777 created "2016-06-24" @default.
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• W2108797777 date "2012-11-05" @default.
• W2108797777 modified "2023-10-09" @default.
• W2108797777 title "Differentiation of isomeric pentose disaccharides by electrospray ionization tandem mass spectrometry and discriminant analysis" @default.
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• W2108797777 doi "https://doi.org/10.1002/rcm.6415" @default.
• W2108797777 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23136020" @default.
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