FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108974251> ?p ?o ?g. }

• W2108974251 endingPage "535" @default.
• W2108974251 startingPage "526" @default.
• W2108974251 abstract "The production and specific productivity of hepatitis B surface antigen (HBsAg) in recombinant Chinese hamster ovary (CHO) cells were increased by 81% and threefold, respectively, when supplemented with 1.5% dimethyl sulfoxide (DMSO) in the culture medium. To investigate the mechanism of DMSO effect on HBsAg production improvement, HBsAg mRNA level was measured by real-time PCR. HBsAg mRNA was increased by about 1.5-fold at 1.5% DMSO. The increase could derive from the increase of HBsAg gene copy number, the improvement of HBsAg mRNA stability, or the acceleration of HBsAg gene transcription. It was found that HBsAg gene copy number was not significantly changed in the cells stimulated with DMSO. HBsAg mRNA stability of cells with DMSO treatment was also not obviously different from control, and the mRNA half-life of 5.58 h in the cells at 1.5% DMSO was comparable to that of 5.36 h in the control culture. DMSO resulted in 80% increase in HBsAg gene transcription activity assessed using a nuclear run-on transcription assay. It could be deduced that the acceleration of HBsAg gene transcription is the main cause of HBsAg production improvement. Biotechnol. Bioeng. 2007;97: 526–535. © 2006 Wiley Periodicals, Inc." @default.
• W2108974251 created "2016-06-24" @default.
• W2108974251 creator A5003551877 @default.
• W2108974251 creator A5020022791 @default.
• W2108974251 creator A5023257111 @default.
• W2108974251 date "2007-01-01" @default.
• W2108974251 modified "2023-09-26" @default.
• W2108974251 title "Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells" @default.
• W2108974251 cites W1971085275 @default.
• W2108974251 cites W1972480731 @default.
• W2108974251 cites W1988010095 @default.
• W2108974251 cites W1990604735 @default.
• W2108974251 cites W1991505994 @default.
• W2108974251 cites W1993223036 @default.
• W2108974251 cites W2005688335 @default.
• W2108974251 cites W2006906242 @default.
• W2108974251 cites W2018047456 @default.
• W2108974251 cites W2029263154 @default.
• W2108974251 cites W2058797471 @default.
• W2108974251 cites W2060873330 @default.
• W2108974251 cites W2062782211 @default.
• W2108974251 cites W2065352348 @default.
• W2108974251 cites W2066799666 @default.
• W2108974251 cites W2080065669 @default.
• W2108974251 cites W2087185866 @default.
• W2108974251 cites W2100187217 @default.
• W2108974251 cites W2107277218 @default.
• W2108974251 cites W2143146123 @default.
• W2108974251 cites W2164578725 @default.
• W2108974251 cites W2170064307 @default.
• W2108974251 cites W270919198 @default.
• W2108974251 cites W302715311 @default.
• W2108974251 doi "https://doi.org/10.1002/bit.21262" @default.
• W2108974251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17099910" @default.
• W2108974251 hasPublicationYear "2007" @default.
• W2108974251 type Work @default.
• W2108974251 sameAs 2108974251 @default.
• W2108974251 citedByCount "21" @default.
• W2108974251 countsByYear W21089742512012 @default.
• W2108974251 countsByYear W21089742512015 @default.
• W2108974251 countsByYear W21089742512017 @default.
• W2108974251 countsByYear W21089742512019 @default.
• W2108974251 countsByYear W21089742512020 @default.
• W2108974251 countsByYear W21089742512021 @default.
• W2108974251 crossrefType "journal-article" @default.
• W2108974251 hasAuthorship W2108974251A5003551877 @default.
• W2108974251 hasAuthorship W2108974251A5020022791 @default.
• W2108974251 hasAuthorship W2108974251A5023257111 @default.
• W2108974251 hasConcept C104317684 @default.
• W2108974251 hasConcept C105580179 @default.
• W2108974251 hasConcept C138885662 @default.
• W2108974251 hasConcept C150194340 @default.
• W2108974251 hasConcept C153911025 @default.
• W2108974251 hasConcept C159047783 @default.
• W2108974251 hasConcept C175656101 @default.
• W2108974251 hasConcept C178790620 @default.
• W2108974251 hasConcept C179926584 @default.
• W2108974251 hasConcept C185592680 @default.
• W2108974251 hasConcept C2522874641 @default.
• W2108974251 hasConcept C2777410769 @default.
• W2108974251 hasConcept C2778452553 @default.
• W2108974251 hasConcept C2780593183 @default.
• W2108974251 hasConcept C41895202 @default.
• W2108974251 hasConcept C54355233 @default.
• W2108974251 hasConcept C55493867 @default.
• W2108974251 hasConcept C81885089 @default.
• W2108974251 hasConcept C86803240 @default.
• W2108974251 hasConceptScore W2108974251C104317684 @default.
• W2108974251 hasConceptScore W2108974251C105580179 @default.
• W2108974251 hasConceptScore W2108974251C138885662 @default.
• W2108974251 hasConceptScore W2108974251C150194340 @default.
• W2108974251 hasConceptScore W2108974251C153911025 @default.
• W2108974251 hasConceptScore W2108974251C159047783 @default.
• W2108974251 hasConceptScore W2108974251C175656101 @default.
• W2108974251 hasConceptScore W2108974251C178790620 @default.
• W2108974251 hasConceptScore W2108974251C179926584 @default.
• W2108974251 hasConceptScore W2108974251C185592680 @default.
• W2108974251 hasConceptScore W2108974251C2522874641 @default.
• W2108974251 hasConceptScore W2108974251C2777410769 @default.
• W2108974251 hasConceptScore W2108974251C2778452553 @default.
• W2108974251 hasConceptScore W2108974251C2780593183 @default.
• W2108974251 hasConceptScore W2108974251C41895202 @default.
• W2108974251 hasConceptScore W2108974251C54355233 @default.
• W2108974251 hasConceptScore W2108974251C55493867 @default.
• W2108974251 hasConceptScore W2108974251C81885089 @default.
• W2108974251 hasConceptScore W2108974251C86803240 @default.
• W2108974251 hasIssue "3" @default.
• W2108974251 hasLocation W21089742511 @default.
• W2108974251 hasLocation W21089742512 @default.
• W2108974251 hasOpenAccess W2108974251 @default.
• W2108974251 hasPrimaryLocation W21089742511 @default.
• W2108974251 hasRelatedWork W1570674964 @default.
• W2108974251 hasRelatedWork W1972541767 @default.
• W2108974251 hasRelatedWork W2005531722 @default.
• W2108974251 hasRelatedWork W2009966535 @default.
• W2108974251 hasRelatedWork W2052545121 @default.
• W2108974251 hasRelatedWork W2053393868 @default.
• W2108974251 hasRelatedWork W2108974251 @default.

• next