FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2108992097> ?p ?o ?g. }

• W2108992097 endingPage "340" @default.
• W2108992097 startingPage "333" @default.
• W2108992097 abstract "A number of complex processes are involved in Eimeria spp. survival, including control of sporulation, intracellular invasion, evasion of host immune responses, successful reproduction, and nutrition. Proteases have been implicated in many of these processes, but the occurrence and functions of serine proteases have not been characterized. Bioinformatic analysis suggests that the Eimeria tenella genome contains several serine proteases that lack homology to trypsin. Using RT-PCR, a gene encoding a subtilisin-like and a rhomboid protease-like serine protease was shown to be developmentally regulated, both being poorly expressed in sporozoites (SZ) and merozoites (MZ). Casein substrate gel electrophoresis of oocyst extracts during sporulation demonstrated bands of proteolytic activity with relative molecular weights (Mr) of 18, 25, and 45 kDa that were eliminated by coincubation with serine protease inhibitors. A protease with Mr of 25 kDa was purified from extracts of unsporulated oocysts by a combination of affinity and anion exchange chromatography. Extracts of SZ contained only a single band of inhibitor-sensitive proteolytic activity at 25 kDa, while the pattern of proteases from extracts of MZ was similar to that of oocysts except for the occurrence of a 90 kDa protease, resistant to protease inhibitors. Excretory-secretory products (ESP) from MZ contained AEBSF (4-[2-Aminoethyl] benzenesulphonyl fluoride)-sensitive protease activity with a specific activity about 10 times greater than that observed in MZ extracts. No protease activity was observed in the ESP from SZ. Pretreatment of SZ with AEBSF significantly reduced SZ invasion and the release of the microneme protein, MIC2. The current results suggest that serine proteases are present in all the developmental stages examined." @default.
• W2108992097 created "2016-06-24" @default.
• W2108992097 creator A5031545480 @default.
• W2108992097 creator A5035053381 @default.
• W2108992097 creator A5070545407 @default.
• W2108992097 creator A5088636426 @default.
• W2108992097 date "2007-04-01" @default.
• W2108992097 modified "2023-10-16" @default.
• W2108992097 title "SERINE PROTEASE ACTIVITY IN DEVELOPMENTAL STAGES OF EIMERIA TENELLA" @default.
• W2108992097 cites W1543727318 @default.
• W2108992097 cites W1984396607 @default.
• W2108992097 cites W1998603390 @default.
• W2108992097 cites W2007160781 @default.
• W2108992097 cites W2008034924 @default.
• W2108992097 cites W2013944635 @default.
• W2108992097 cites W2014655116 @default.
• W2108992097 cites W2015887847 @default.
• W2108992097 cites W2021018772 @default.
• W2108992097 cites W2042683294 @default.
• W2108992097 cites W2059696825 @default.
• W2108992097 cites W2061149028 @default.
• W2108992097 cites W2066104894 @default.
• W2108992097 cites W2072843074 @default.
• W2108992097 cites W2079577669 @default.
• W2108992097 cites W2082365218 @default.
• W2108992097 cites W2093020034 @default.
• W2108992097 cites W2135743054 @default.
• W2108992097 cites W2142327485 @default.
• W2108992097 cites W2147552637 @default.
• W2108992097 cites W2152478355 @default.
• W2108992097 cites W2152630125 @default.
• W2108992097 cites W2156352436 @default.
• W2108992097 cites W2158714788 @default.
• W2108992097 cites W2161457319 @default.
• W2108992097 cites W2165237462 @default.
• W2108992097 cites W2166668583 @default.
• W2108992097 cites W2180026370 @default.
• W2108992097 cites W4238052567 @default.
• W2108992097 doi "https://doi.org/10.1645/ge-824r1.1" @default.
• W2108992097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17539417" @default.
• W2108992097 hasPublicationYear "2007" @default.
• W2108992097 type Work @default.
• W2108992097 sameAs 2108992097 @default.
• W2108992097 citedByCount "16" @default.
• W2108992097 countsByYear W21089920972012 @default.
• W2108992097 countsByYear W21089920972015 @default.
• W2108992097 countsByYear W21089920972016 @default.
• W2108992097 countsByYear W21089920972021 @default.
• W2108992097 countsByYear W21089920972022 @default.
• W2108992097 countsByYear W21089920972023 @default.
• W2108992097 crossrefType "journal-article" @default.
• W2108992097 hasAuthorship W2108992097A5031545480 @default.
• W2108992097 hasAuthorship W2108992097A5035053381 @default.
• W2108992097 hasAuthorship W2108992097A5070545407 @default.
• W2108992097 hasAuthorship W2108992097A5088636426 @default.
• W2108992097 hasConcept C142462285 @default.
• W2108992097 hasConcept C153911025 @default.
• W2108992097 hasConcept C159047783 @default.
• W2108992097 hasConcept C181199279 @default.
• W2108992097 hasConcept C182220744 @default.
• W2108992097 hasConcept C2522874641 @default.
• W2108992097 hasConcept C2775883766 @default.
• W2108992097 hasConcept C2776414213 @default.
• W2108992097 hasConcept C2776714187 @default.
• W2108992097 hasConcept C2777807008 @default.
• W2108992097 hasConcept C2777976947 @default.
• W2108992097 hasConcept C2779811187 @default.
• W2108992097 hasConcept C2781143361 @default.
• W2108992097 hasConcept C2993143319 @default.
• W2108992097 hasConcept C55493867 @default.
• W2108992097 hasConcept C82553315 @default.
• W2108992097 hasConcept C86803240 @default.
• W2108992097 hasConcept C89423630 @default.
• W2108992097 hasConceptScore W2108992097C142462285 @default.
• W2108992097 hasConceptScore W2108992097C153911025 @default.
• W2108992097 hasConceptScore W2108992097C159047783 @default.
• W2108992097 hasConceptScore W2108992097C181199279 @default.
• W2108992097 hasConceptScore W2108992097C182220744 @default.
• W2108992097 hasConceptScore W2108992097C2522874641 @default.
• W2108992097 hasConceptScore W2108992097C2775883766 @default.
• W2108992097 hasConceptScore W2108992097C2776414213 @default.
• W2108992097 hasConceptScore W2108992097C2776714187 @default.
• W2108992097 hasConceptScore W2108992097C2777807008 @default.
• W2108992097 hasConceptScore W2108992097C2777976947 @default.
• W2108992097 hasConceptScore W2108992097C2779811187 @default.
• W2108992097 hasConceptScore W2108992097C2781143361 @default.
• W2108992097 hasConceptScore W2108992097C2993143319 @default.
• W2108992097 hasConceptScore W2108992097C55493867 @default.
• W2108992097 hasConceptScore W2108992097C82553315 @default.
• W2108992097 hasConceptScore W2108992097C86803240 @default.
• W2108992097 hasConceptScore W2108992097C89423630 @default.
• W2108992097 hasIssue "2" @default.
• W2108992097 hasLocation W21089920971 @default.
• W2108992097 hasLocation W21089920972 @default.
• W2108992097 hasOpenAccess W2108992097 @default.
• W2108992097 hasPrimaryLocation W21089920971 @default.
• W2108992097 hasRelatedWork W1976128446 @default.
• W2108992097 hasRelatedWork W2026960302 @default.

• next