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• W2109010031 abstract "It has been reported that nuclei from HeLa cells are responsible for approximately 10%-15% of total cellular protein synthesis. We show here that isolated Chinese hamster ovary (CHO) and HeLa cell nuclei are essentially inactive for translation, and that the earlier results were most likely due to cytoplasmic contamination. Moreover, we suggest that the nascent polypeptides observed in nuclei of permeabilized cells may have been due to overpermeabilization and consequent damage to the cells. Based on this information, we conclude that nuclear protein synthesis, if it exists, is limited to less than 1% of that in cells." @default.
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• W2109010031 date "2003-01-01" @default.
• W2109010031 modified "2023-10-16" @default.
• W2109010031 title "Nuclear protein synthesis: A re-evaluation" @default.
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• W2109010031 doi "https://doi.org/10.1261/rna.2990203" @default.
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