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• W2109062292 endingPage "1154" @default.
• W2109062292 startingPage "1148" @default.
• W2109062292 abstract "ABSTRACT Amplified fragment length polymorphism (AFLP) analysis was applied to characterize 33 group I and 37 group II Clostridium botulinum strains. Four restriction enzyme and 30 primer combinations were screened to tailor the AFLP technique for optimal characterization of C. botulinum . The enzyme combination HindIII and HpyCH4IV, with primers having one selective nucleotide apiece (Hind-C and Hpy-A), was selected. AFLP clearly differentiated between C. botulinum groups I and II; group-specific clusters showed <10% similarity between proteolytic and nonproteolytic C. botulinum strains. In addition, group-specific fragments were detected in both groups. All strains studied were typeable by AFLP, and a total of 42 AFLP types were identified. Extensive diversity was observed among strains of C. botulinum type E, whereas group I had lower genetic biodiversity. These results indicate that AFLP is a fast, highly discriminating, and reproducible DNA fingerprinting method with excellent typeability, which, in addition to its suitability for typing at strain level, can be used for C. botulinum group identification." @default.
• W2109062292 created "2016-06-24" @default.
• W2109062292 creator A5008901078 @default.
• W2109062292 creator A5029139884 @default.
• W2109062292 creator A5049591633 @default.
• W2109062292 date "2005-03-01" @default.
• W2109062292 modified "2023-09-23" @default.
• W2109062292 title "Efficient DNA Fingerprinting of <i>Clostridium botulinum</i> Types A, B, E, and F by Amplified Fragment Length Polymorphism Analysis" @default.
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• W2109062292 doi "https://doi.org/10.1128/aem.71.3.1148-1154.2005" @default.
• W2109062292 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1065150" @default.
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