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• W2109092774 abstract "The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1.A pair of primers was designed and used to amplify a 119 bp DNA fragment to generate a recombinant plasmid which was served as the standard. A SYBR I qPCR protocol was established using the P1 recombinant plasmid standard and the sensitivity, specificity and stability of this method was analyzed. The results demonstrate a strong correlation with P1 recombinant plasmid titers when virus DNA copy numbers fall in between 10(0) ~ 10(9) copies/μL. This method doesn't detect pseudo rabies, porcine parvovirus or porcine reproductive and respiratory syndrome virus; moreover it can distinguish porcine circovirus type 2 from P1 by melting temperature analysis. Coefficient of variation for each batch of reaction is less than 5%. The serum virus titers of P1 positive in this study were measured by this protocol to be 10(3) to 10(7) copies/mL.The established qPCR is sensitive, specific, and reliable, which could be a useful tool when applied to quantification of P1 in a variety of samples from infected pigs." @default.
• W2109092774 created "2016-06-24" @default.
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• W2109092774 date "2015-09-24" @default.
• W2109092774 modified "2023-10-18" @default.
• W2109092774 title "Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China" @default.
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• W2109092774 doi "https://doi.org/10.1186/s12917-015-0509-3" @default.
• W2109092774 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4583164" @default.
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