Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109103393> ?p ?o ?g. }
- W2109103393 endingPage "468" @default.
- W2109103393 startingPage "456" @default.
- W2109103393 abstract "Retroviral integration involves two DNA substrates that play different roles. The viral DNA substrate is recognized by virtue of specific nucleotide sequences near the end of a double-stranded DNA molecule. The target DNA substrate is recognized at internal sites with little sequence preference; nucleosomal DNA appears to be preferred for this role. Despite this apparent asymmetry in the sequence, structure, and roles of the DNA substrates in the integration reaction, the existence of distinct binding sites for viral and target DNA substrates has been controversial. In this report, we describe the expression in Escherichia coli and purification of Moloney murine leukemia virus integrase as a fusion protein with glutathione S-transferase, characterization of its activity by using several model DNA substrates, and the initial kinetic characterization of its interactions with a model viral DNA substrate. We provide evidence for functionally and kinetically distinct binding sites for viral and target DNA substrates and describe a cross-linking assay for DNA binding at a site whose specificity is consistent with the target DNA binding site." @default.
- W2109103393 created "2016-06-24" @default.
- W2109103393 creator A5029273084 @default.
- W2109103393 creator A5063172780 @default.
- W2109103393 creator A5065906655 @default.
- W2109103393 creator A5086694164 @default.
- W2109103393 date "1995-01-01" @default.
- W2109103393 modified "2023-09-23" @default.
- W2109103393 title "Characterization of recombinant murine leukemia virus integrase" @default.
- W2109103393 cites W1495877624 @default.
- W2109103393 cites W1502533342 @default.
- W2109103393 cites W1520944136 @default.
- W2109103393 cites W1596343071 @default.
- W2109103393 cites W1616160618 @default.
- W2109103393 cites W1616413236 @default.
- W2109103393 cites W1621406154 @default.
- W2109103393 cites W1685289055 @default.
- W2109103393 cites W1720727101 @default.
- W2109103393 cites W1763828525 @default.
- W2109103393 cites W18475619 @default.
- W2109103393 cites W1939718347 @default.
- W2109103393 cites W1945985833 @default.
- W2109103393 cites W1960707077 @default.
- W2109103393 cites W1966815449 @default.
- W2109103393 cites W1971994381 @default.
- W2109103393 cites W1979745608 @default.
- W2109103393 cites W1982707123 @default.
- W2109103393 cites W1992073293 @default.
- W2109103393 cites W1999686757 @default.
- W2109103393 cites W2002907404 @default.
- W2109103393 cites W2005663970 @default.
- W2109103393 cites W2008666823 @default.
- W2109103393 cites W2014568295 @default.
- W2109103393 cites W2018289835 @default.
- W2109103393 cites W2021650808 @default.
- W2109103393 cites W2025260589 @default.
- W2109103393 cites W2026184248 @default.
- W2109103393 cites W2041126567 @default.
- W2109103393 cites W2050569727 @default.
- W2109103393 cites W2055995901 @default.
- W2109103393 cites W2058019531 @default.
- W2109103393 cites W2059201099 @default.
- W2109103393 cites W2065979160 @default.
- W2109103393 cites W2068446924 @default.
- W2109103393 cites W2073565105 @default.
- W2109103393 cites W2075336365 @default.
- W2109103393 cites W2084278833 @default.
- W2109103393 cites W2089882643 @default.
- W2109103393 cites W2095816327 @default.
- W2109103393 cites W2097179700 @default.
- W2109103393 cites W2100837269 @default.
- W2109103393 cites W2105648514 @default.
- W2109103393 cites W2107777256 @default.
- W2109103393 cites W2110661303 @default.
- W2109103393 cites W2128635872 @default.
- W2109103393 cites W2132417094 @default.
- W2109103393 cites W2133140574 @default.
- W2109103393 cites W2138149981 @default.
- W2109103393 cites W2147466111 @default.
- W2109103393 cites W2158733225 @default.
- W2109103393 cites W2166782710 @default.
- W2109103393 doi "https://doi.org/10.1128/jvi.69.1.456-468.1995" @default.
- W2109103393 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/188594" @default.
- W2109103393 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7983742" @default.
- W2109103393 hasPublicationYear "1995" @default.
- W2109103393 type Work @default.
- W2109103393 sameAs 2109103393 @default.
- W2109103393 citedByCount "35" @default.
- W2109103393 countsByYear W21091033932012 @default.
- W2109103393 countsByYear W21091033932013 @default.
- W2109103393 crossrefType "journal-article" @default.
- W2109103393 hasAuthorship W2109103393A5029273084 @default.
- W2109103393 hasAuthorship W2109103393A5063172780 @default.
- W2109103393 hasAuthorship W2109103393A5065906655 @default.
- W2109103393 hasAuthorship W2109103393A5086694164 @default.
- W2109103393 hasBestOaLocation W21091033931 @default.
- W2109103393 hasConcept C101762097 @default.
- W2109103393 hasConcept C104317684 @default.
- W2109103393 hasConcept C107824862 @default.
- W2109103393 hasConcept C14430832 @default.
- W2109103393 hasConcept C150194340 @default.
- W2109103393 hasConcept C153911025 @default.
- W2109103393 hasConcept C159047783 @default.
- W2109103393 hasConcept C167625842 @default.
- W2109103393 hasConcept C19924922 @default.
- W2109103393 hasConcept C203284983 @default.
- W2109103393 hasConcept C2522874641 @default.
- W2109103393 hasConcept C2779562191 @default.
- W2109103393 hasConcept C3662595 @default.
- W2109103393 hasConcept C40767141 @default.
- W2109103393 hasConcept C552990157 @default.
- W2109103393 hasConcept C55493867 @default.
- W2109103393 hasConcept C86803240 @default.
- W2109103393 hasConceptScore W2109103393C101762097 @default.
- W2109103393 hasConceptScore W2109103393C104317684 @default.
- W2109103393 hasConceptScore W2109103393C107824862 @default.
- W2109103393 hasConceptScore W2109103393C14430832 @default.
- W2109103393 hasConceptScore W2109103393C150194340 @default.