FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109107385> ?p ?o ?g. }

• W2109107385 endingPage "339" @default.
• W2109107385 startingPage "329" @default.
• W2109107385 abstract "Human alpha-galactosidase A (alpha-Gal A) is the lysosomal glycohydrolase that cleaves the terminal alpha-galactosyl moieties of various glycoconjugates. Overexpression of the enzyme in Chinese hamster ovary (CHO) cells results in high intracellular enzyme accumulation and the selective secretion of active enzyme. Structural analysis of the N -linked oligosaccharides of the intracellular and secreted glycoforms revealed that the secreted enzyme's oligosaccharides were remarkably heterogeneous, having high mannose (63%), complex (30%), and hybrid (5%) structures. The major high mannose oligosaccharides were Man5-7GlcNAc2 species. Approximately 40% of the high mannose and 30% of the hybrid oligosaccharides had phosphate monoester groups. The complex oligosaccharides were mono-, bi-, 2,4-tri-, 2,6-tri- and tetraantennary with or without core-region fucose, many of which had incomplete outer chains. Approximately 30% of the complex oligosaccharides were mono- or disialylated. Sialic acids were mostly N -acetylneuraminic acid and occurred exclusively in alpha2, 3-linkage. In contrast, the intracellular enzyme had only small amounts of complex chains (7.7%) and had predominantly high mannose oligosaccharides (92%), mostly Man5GlcNAc2 and smaller species, of which only 3% were phosphorylated. The complex oligosaccharides were fucosylated and had the same antennary structures as the secreted enzyme. Although most had mature outer chains, none were sialylated. Thus, the overexpression of human alpha-Gal A in CHO cells resulted in different oligosaccharide structures on the secreted and intracellular glycoforms, the highly heterogeneous secreted forms presumably due to the high level expression and impaired glycosylation in the trans- Golgi network, and the predominately Man5-7GlcNAc2 cellular glycoforms resulting from carbohydrate trimming in the lysosome." @default.
• W2109107385 created "2016-06-24" @default.
• W2109107385 creator A5035205865 @default.
• W2109107385 creator A5050033331 @default.
• W2109107385 creator A5084723393 @default.
• W2109107385 creator A5090900773 @default.
• W2109107385 date "1998-04-01" @default.
• W2109107385 modified "2023-10-13" @default.
• W2109107385 title "Human -galactosidase A: characterization of the N-linked oligosaccharides on the intracellular and secreted glycoforms overexpressed by Chinese hamster ovary cells" @default.
• W2109107385 cites W1485462441 @default.
• W2109107385 cites W1490194415 @default.
• W2109107385 cites W1491723881 @default.
• W2109107385 cites W1529968415 @default.
• W2109107385 cites W1534176709 @default.
• W2109107385 cites W1550357952 @default.
• W2109107385 cites W1561511774 @default.
• W2109107385 cites W1568892768 @default.
• W2109107385 cites W1570049651 @default.
• W2109107385 cites W1570484367 @default.
• W2109107385 cites W1595400617 @default.
• W2109107385 cites W1602072520 @default.
• W2109107385 cites W1761811954 @default.
• W2109107385 cites W1963920970 @default.
• W2109107385 cites W1964112957 @default.
• W2109107385 cites W1964980418 @default.
• W2109107385 cites W1966112920 @default.
• W2109107385 cites W1970844016 @default.
• W2109107385 cites W1974917902 @default.
• W2109107385 cites W1983204381 @default.
• W2109107385 cites W1983299727 @default.
• W2109107385 cites W2024878197 @default.
• W2109107385 cites W2033081167 @default.
• W2109107385 cites W2033287927 @default.
• W2109107385 cites W2037485073 @default.
• W2109107385 cites W2054139356 @default.
• W2109107385 cites W2061288769 @default.
• W2109107385 cites W2066068990 @default.
• W2109107385 cites W2075869038 @default.
• W2109107385 cites W2076311962 @default.
• W2109107385 cites W2078379563 @default.
• W2109107385 cites W2094154860 @default.
• W2109107385 cites W2124376994 @default.
• W2109107385 cites W2155104104 @default.
• W2109107385 cites W2201716983 @default.
• W2109107385 cites W2212445342 @default.
• W2109107385 cites W2261677824 @default.
• W2109107385 cites W2318566832 @default.
• W2109107385 doi "https://doi.org/10.1093/glycob/8.4.329" @default.
• W2109107385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9499380" @default.
• W2109107385 hasPublicationYear "1998" @default.
• W2109107385 type Work @default.
• W2109107385 sameAs 2109107385 @default.
• W2109107385 citedByCount "47" @default.
• W2109107385 countsByYear W21091073852012 @default.
• W2109107385 countsByYear W21091073852013 @default.
• W2109107385 countsByYear W21091073852015 @default.
• W2109107385 countsByYear W21091073852016 @default.
• W2109107385 countsByYear W21091073852017 @default.
• W2109107385 countsByYear W21091073852018 @default.
• W2109107385 countsByYear W21091073852019 @default.
• W2109107385 countsByYear W21091073852020 @default.
• W2109107385 countsByYear W21091073852021 @default.
• W2109107385 countsByYear W21091073852022 @default.
• W2109107385 countsByYear W21091073852023 @default.
• W2109107385 crossrefType "journal-article" @default.
• W2109107385 hasAuthorship W2109107385A5035205865 @default.
• W2109107385 hasAuthorship W2109107385A5050033331 @default.
• W2109107385 hasAuthorship W2109107385A5084723393 @default.
• W2109107385 hasAuthorship W2109107385A5090900773 @default.
• W2109107385 hasBestOaLocation W21091073851 @default.
• W2109107385 hasConcept C108625454 @default.
• W2109107385 hasConcept C119062480 @default.
• W2109107385 hasConcept C120694314 @default.
• W2109107385 hasConcept C13426554 @default.
• W2109107385 hasConcept C158617107 @default.
• W2109107385 hasConcept C170493617 @default.
• W2109107385 hasConcept C175656101 @default.
• W2109107385 hasConcept C181199279 @default.
• W2109107385 hasConcept C185592680 @default.
• W2109107385 hasConcept C206212055 @default.
• W2109107385 hasConcept C2775887612 @default.
• W2109107385 hasConcept C2776841590 @default.
• W2109107385 hasConcept C2777313579 @default.
• W2109107385 hasConcept C2777726330 @default.
• W2109107385 hasConcept C2780115458 @default.
• W2109107385 hasConcept C2780216420 @default.
• W2109107385 hasConcept C49039625 @default.
• W2109107385 hasConcept C55493867 @default.
• W2109107385 hasConcept C79879829 @default.
• W2109107385 hasConcept C86803240 @default.
• W2109107385 hasConceptScore W2109107385C108625454 @default.
• W2109107385 hasConceptScore W2109107385C119062480 @default.
• W2109107385 hasConceptScore W2109107385C120694314 @default.
• W2109107385 hasConceptScore W2109107385C13426554 @default.
• W2109107385 hasConceptScore W2109107385C158617107 @default.
• W2109107385 hasConceptScore W2109107385C170493617 @default.
• W2109107385 hasConceptScore W2109107385C175656101 @default.
• W2109107385 hasConceptScore W2109107385C181199279 @default.

• next