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• W2109170986 abstract "Two primary and two metastatic cell lines with distinct phenotypes and genotypes have been established from a patient diagnosed as having infiltrating and intraductal mammary carcinoma (21T series). All four lines can be cultured in the same medium, DFCI-1, which also supports long-term growth of normal epithelial cells. Therefore, we have been able to compare normal and tumor cells at the cellular and molecular levels. The mammary origin of the 21T series was confirmed by using antibodies against the human milk fat globule antigen-2 epitope. The two primary tumor lines (21NT and 21PT) are both immortal and aneuploid, although only 21NT is tumorigenic in the nude mouse assay. The two populations derived from the metastatic pleural effusion (21MT-1 and 21MT-2) each exhibit distinct characteristics in morphology and growth factor requirements. The erbB2 gene is amplified and overexpressed in all of these cell lines compared to normal epithelial cell controls. These four tumor cell lines from a single patient represent a mammary tumor progression series that has been established in long-term cell culture." @default.
• W2109170986 created "2016-06-24" @default.
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• W2109170986 date "1990-11-15" @default.
• W2109170986 modified "2023-09-27" @default.
• W2109170986 title "Tumor progression in four mammary epithelial cell lines derived from the same patient." @default.
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• W2109170986 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1977518" @default.
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