Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109181288> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2109181288 endingPage "426" @default.
- W2109181288 startingPage "423" @default.
- W2109181288 abstract "Reporter genes are often used to monitor gene expression in various organisms. To investigate the use of a dual-color luciferase reporter assay (DCRA) for monitoring expression of multiple genes in higher plants, we characterized the bioluminescence and the spectra of click beetle luciferases in planta. The DCRA consists of click beetle green luciferase (CBG68luc) and click beetle red luciferase (CBRluc). The green and the red bioluminescence from CBG68luc and CBRluc, respectively, are suitable for nondestructive in vivo assays because luminescence signals can be monitored selectively using optical filters. Using transgenic plants and a transient expression system, we introduced and expressed CBG68luc and CBRluc reporter genes in plant cells and obtained luminescence spectra by spectrometry. The spectral patterns from the transgenic plant cells were similar to those from E. coli transformed with the same reporter genes. Although plant tissues contain pigments and autofluorescent materials, our data suggest that the presence of these do not interfere with gene expression monitoring by DCRA, which is therefore applicable to monitoring the regulation of gene expression in plants." @default.
- W2109181288 created "2016-06-24" @default.
- W2109181288 creator A5014111711 @default.
- W2109181288 creator A5023362638 @default.
- W2109181288 creator A5023515473 @default.
- W2109181288 date "2011-01-01" @default.
- W2109181288 modified "2023-09-27" @default.
- W2109181288 title "Bioluminescence spectra of click beetle luciferases in higher plant cells" @default.
- W2109181288 cites W1973305463 @default.
- W2109181288 cites W1978406742 @default.
- W2109181288 cites W1991988050 @default.
- W2109181288 cites W1992033675 @default.
- W2109181288 cites W1998763797 @default.
- W2109181288 cites W2024556021 @default.
- W2109181288 cites W2025216143 @default.
- W2109181288 cites W2028306503 @default.
- W2109181288 cites W2095846115 @default.
- W2109181288 cites W2135951009 @default.
- W2109181288 cites W2163031963 @default.
- W2109181288 doi "https://doi.org/10.5511/plantbiotechnology.11.0804a" @default.
- W2109181288 hasPublicationYear "2011" @default.
- W2109181288 type Work @default.
- W2109181288 sameAs 2109181288 @default.
- W2109181288 citedByCount "2" @default.
- W2109181288 countsByYear W21091812882012 @default.
- W2109181288 countsByYear W21091812882020 @default.
- W2109181288 crossrefType "journal-article" @default.
- W2109181288 hasAuthorship W2109181288A5014111711 @default.
- W2109181288 hasAuthorship W2109181288A5023362638 @default.
- W2109181288 hasAuthorship W2109181288A5023515473 @default.
- W2109181288 hasBestOaLocation W21091812881 @default.
- W2109181288 hasConcept C102230213 @default.
- W2109181288 hasConcept C104317684 @default.
- W2109181288 hasConcept C111335760 @default.
- W2109181288 hasConcept C150194340 @default.
- W2109181288 hasConcept C153911025 @default.
- W2109181288 hasConcept C161733203 @default.
- W2109181288 hasConcept C161997846 @default.
- W2109181288 hasConcept C173764188 @default.
- W2109181288 hasConcept C2909536089 @default.
- W2109181288 hasConcept C5176359 @default.
- W2109181288 hasConcept C54009773 @default.
- W2109181288 hasConcept C55493867 @default.
- W2109181288 hasConcept C86803240 @default.
- W2109181288 hasConceptScore W2109181288C102230213 @default.
- W2109181288 hasConceptScore W2109181288C104317684 @default.
- W2109181288 hasConceptScore W2109181288C111335760 @default.
- W2109181288 hasConceptScore W2109181288C150194340 @default.
- W2109181288 hasConceptScore W2109181288C153911025 @default.
- W2109181288 hasConceptScore W2109181288C161733203 @default.
- W2109181288 hasConceptScore W2109181288C161997846 @default.
- W2109181288 hasConceptScore W2109181288C173764188 @default.
- W2109181288 hasConceptScore W2109181288C2909536089 @default.
- W2109181288 hasConceptScore W2109181288C5176359 @default.
- W2109181288 hasConceptScore W2109181288C54009773 @default.
- W2109181288 hasConceptScore W2109181288C55493867 @default.
- W2109181288 hasConceptScore W2109181288C86803240 @default.
- W2109181288 hasIssue "4" @default.
- W2109181288 hasLocation W21091812881 @default.
- W2109181288 hasOpenAccess W2109181288 @default.
- W2109181288 hasPrimaryLocation W21091812881 @default.
- W2109181288 hasRelatedWork W2068177630 @default.
- W2109181288 hasRelatedWork W2095111437 @default.
- W2109181288 hasRelatedWork W2109181288 @default.
- W2109181288 hasRelatedWork W2131360889 @default.
- W2109181288 hasRelatedWork W2801483177 @default.
- W2109181288 hasRelatedWork W2980263833 @default.
- W2109181288 hasRelatedWork W2982739013 @default.
- W2109181288 hasRelatedWork W2999853337 @default.
- W2109181288 hasRelatedWork W3115463216 @default.
- W2109181288 hasRelatedWork W3136826821 @default.
- W2109181288 hasVolume "28" @default.
- W2109181288 isParatext "false" @default.
- W2109181288 isRetracted "false" @default.
- W2109181288 magId "2109181288" @default.
- W2109181288 workType "article" @default.