Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109183119> ?p ?o ?g. }
- W2109183119 endingPage "739" @default.
- W2109183119 startingPage "726" @default.
- W2109183119 abstract "The Escherichia coli MnmE protein is a 50-kDa multidomain GTPase involved in tRNA modification. Its homologues in eukaryotes are crucial for mitochondrial respiration and, thus, it is thought that the human protein might be involved in mitochondrial diseases. Unlike Ras, MnmE shows a high intrinsic GTPase activity and requires effective GTP hydrolysis, and not simply GTP binding, to be functionally active. The isolated MnmE G-domain (165 residues) conserves the GTPase activity of the entire protein, suggesting that it contains the catalytic residues for GTP hydrolysis. To explore the GTP hydrolysis mechanism of MnmE, we analyzed the effect of low pH on binding and hydrolysis of GTP, as well as on the formation of a MnmE transition state mimic. GTP hydrolysis by MnmE, but not GTP binding or formation of a complex with mant-GDP and aluminium fluoride, is impaired at acidic pH, suggesting that the chemistry of the transition state mimic is different to that of the true transition state, and that some residue(s), critical for GTP hydrolysis, is severely affected by low pH. We use a nuclear magnetic resonance (NMR)-based approach to get insights into the MnmE structure and properties. The combined use of NMR restraints and homology structural information allowed the determination of the MnmE G-domain structure in its free form. Chemical shift structure-based prediction provided a good basis for structure refinement and validation. Our data support that MnmE, unlike other GTPases, does not use an arginine finger to drive catalysis, although Arg252 may play a role in stabilization of the transition state. Proteins 2007. © 2006 Wiley-Liss, Inc." @default.
- W2109183119 created "2016-06-24" @default.
- W2109183119 creator A5001808454 @default.
- W2109183119 creator A5054024923 @default.
- W2109183119 creator A5056798781 @default.
- W2109183119 creator A5075785081 @default.
- W2109183119 creator A5077332986 @default.
- W2109183119 creator A5077584810 @default.
- W2109183119 creator A5086861413 @default.
- W2109183119 date "2006-12-01" @default.
- W2109183119 modified "2023-10-18" @default.
- W2109183119 title "Structural insights into the GTPase domain of Escherichia coli MnmE protein" @default.
- W2109183119 cites W1499450468 @default.
- W2109183119 cites W1589295940 @default.
- W2109183119 cites W1595347157 @default.
- W2109183119 cites W1672842817 @default.
- W2109183119 cites W1969467512 @default.
- W2109183119 cites W1970052480 @default.
- W2109183119 cites W1973916295 @default.
- W2109183119 cites W1974607992 @default.
- W2109183119 cites W1975459052 @default.
- W2109183119 cites W1977598234 @default.
- W2109183119 cites W1980467950 @default.
- W2109183119 cites W1988257152 @default.
- W2109183119 cites W1992864103 @default.
- W2109183119 cites W1995017064 @default.
- W2109183119 cites W2001757975 @default.
- W2109183119 cites W2002195659 @default.
- W2109183119 cites W2008708467 @default.
- W2109183119 cites W2014713866 @default.
- W2109183119 cites W2022046854 @default.
- W2109183119 cites W2024027456 @default.
- W2109183119 cites W2026649417 @default.
- W2109183119 cites W2030788867 @default.
- W2109183119 cites W2045322789 @default.
- W2109183119 cites W2047384586 @default.
- W2109183119 cites W2047627595 @default.
- W2109183119 cites W2052933818 @default.
- W2109183119 cites W2056668858 @default.
- W2109183119 cites W2065283382 @default.
- W2109183119 cites W2065735398 @default.
- W2109183119 cites W2077167882 @default.
- W2109183119 cites W2080467264 @default.
- W2109183119 cites W2088112980 @default.
- W2109183119 cites W2090198616 @default.
- W2109183119 cites W2091803996 @default.
- W2109183119 cites W2095642827 @default.
- W2109183119 cites W2101574921 @default.
- W2109183119 cites W2104480986 @default.
- W2109183119 cites W2106882534 @default.
- W2109183119 cites W2117006495 @default.
- W2109183119 cites W2139723407 @default.
- W2109183119 cites W2151639191 @default.
- W2109183119 cites W2151920771 @default.
- W2109183119 cites W2156571424 @default.
- W2109183119 cites W2158714788 @default.
- W2109183119 cites W2165552051 @default.
- W2109183119 doi "https://doi.org/10.1002/prot.21186" @default.
- W2109183119 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17143896" @default.
- W2109183119 hasPublicationYear "2006" @default.
- W2109183119 type Work @default.
- W2109183119 sameAs 2109183119 @default.
- W2109183119 citedByCount "5" @default.
- W2109183119 countsByYear W21091831192013 @default.
- W2109183119 countsByYear W21091831192022 @default.
- W2109183119 crossrefType "journal-article" @default.
- W2109183119 hasAuthorship W2109183119A5001808454 @default.
- W2109183119 hasAuthorship W2109183119A5054024923 @default.
- W2109183119 hasAuthorship W2109183119A5056798781 @default.
- W2109183119 hasAuthorship W2109183119A5075785081 @default.
- W2109183119 hasAuthorship W2109183119A5077332986 @default.
- W2109183119 hasAuthorship W2109183119A5077584810 @default.
- W2109183119 hasAuthorship W2109183119A5086861413 @default.
- W2109183119 hasConcept C118716 @default.
- W2109183119 hasConcept C12554922 @default.
- W2109183119 hasConcept C181199279 @default.
- W2109183119 hasConcept C185592680 @default.
- W2109183119 hasConcept C207332259 @default.
- W2109183119 hasConcept C2776638253 @default.
- W2109183119 hasConcept C47701112 @default.
- W2109183119 hasConcept C55493867 @default.
- W2109183119 hasConcept C86803240 @default.
- W2109183119 hasConcept C94412978 @default.
- W2109183119 hasConceptScore W2109183119C118716 @default.
- W2109183119 hasConceptScore W2109183119C12554922 @default.
- W2109183119 hasConceptScore W2109183119C181199279 @default.
- W2109183119 hasConceptScore W2109183119C185592680 @default.
- W2109183119 hasConceptScore W2109183119C207332259 @default.
- W2109183119 hasConceptScore W2109183119C2776638253 @default.
- W2109183119 hasConceptScore W2109183119C47701112 @default.
- W2109183119 hasConceptScore W2109183119C55493867 @default.
- W2109183119 hasConceptScore W2109183119C86803240 @default.
- W2109183119 hasConceptScore W2109183119C94412978 @default.
- W2109183119 hasIssue "3" @default.
- W2109183119 hasLocation W21091831191 @default.
- W2109183119 hasLocation W21091831192 @default.
- W2109183119 hasOpenAccess W2109183119 @default.
- W2109183119 hasPrimaryLocation W21091831191 @default.