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- W2109201412 abstract "ABSTRACT In this study, we have isolated a temperate phage (ΦCD119) from a pathogenic Clostridium difficile strain and sequenced and annotated its genome. This virus has an icosahedral capsid and a contractile tail covered by a sheath and contains a double-stranded DNA genome. It belongs to the Myoviridae family of the tailed phages and the order Caudovirales . The genome was circularly permuted, with no physical ends detected by sequencing or restriction enzyme digestion analysis, and lacked a cos site. The DNA sequence of this phage consists of 53,325 bp, which carries 79 putative open reading frames (ORFs). A function could be assigned to 23 putative gene products, based upon bioinformatic analyses. The ΦCD119 genome is organized in a modular format, which includes modules for lysogeny, DNA replication, DNA packaging, structural proteins, and host cell lysis. The ΦCD119 attachment site attP lies in a noncoding region close to the putative integrase ( int ) gene. We have identified the phage integration site on the C. difficile chromosome ( attB ) located in a noncoding region just upstream of gene gltP , which encodes a carrier protein for glutamate and aspartate. This genetic analysis represents the first complete DNA sequence and annotation of a C. difficile phage." @default.
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- W2109201412 date "2006-04-01" @default.
- W2109201412 modified "2023-10-14" @default.
- W2109201412 title "Genomic Organization and Molecular Characterization of <i>Clostridium difficile</i> Bacteriophage ΦCD119" @default.
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- W2109201412 doi "https://doi.org/10.1128/jb.188.7.2568-2577.2006" @default.
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