FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109241881> ?p ?o ?g. }

• W2109241881 endingPage "575" @default.
• W2109241881 startingPage "567" @default.
• W2109241881 abstract "This article describes the step-wise approach undertaken to select a serum-free medium (SFM) for the efficient production of a recombinant adenoviral vectors expressing beta-galactosidase (Ad5 CMV-LacZ), in the complementing human embryonic kidney 293S cells. In the first step, a 293S-derived transfectoma, secreting a soluble epidermal growth factor receptor sEGFr (D2-22), was used to estimate the potential of selected serum-free formulations to support the production of a recombinant protein as compared to serum-containing medium. Assays showed that only one among six commercial serum-free formulations could support both sEGFr production and cell growth in static or suspension culture. In commercially available calcium-containing serum-free formulations, the cell aggregates reached up to 3 mm in diameter. In the second step, 293S cells were gradually adapted to a low-calcium version of the selected medium (LC-SFM). Cells were cloned, then screened according to their ability to grow at a rate and an extent comparable to parental cells in serum-containing medium (standard) as single cells or small aggregates. The 293SF-3F6 clone, first adapted to and then cloned in the selected serum-free medium, was selected for further experiments. Bioreactor run performed with the 293SF-3F6 clone showed similar growth curve as in the shake-flask controls. In the final step, the recombinant viral vector productivity of the 293S cells and the 293SF-3F6 clone was tested. The 293SF-3F6 cells infected by Ad5 CMV-LacZ in 3 L-scale bioreactor maintained the specific productivities of both beta-galactosidase and adenoviral vector equivalent to the shake-flask controls in suspension culture. Results from this study clearly demonstrate that the 293SF-3F6 cell line thus selected may be used either for establishing stable transfected cell line or for the production of adenoviral vectors required for gene therapy studies." @default.
• W2109241881 created "2016-06-24" @default.
• W2109241881 creator A5004289043 @default.
• W2109241881 creator A5013737653 @default.
• W2109241881 creator A5069297606 @default.
• W2109241881 creator A5079295525 @default.
• W2109241881 date "1998-09-05" @default.
• W2109241881 modified "2023-10-10" @default.
• W2109241881 title "Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells" @default.
• W2109241881 cites W1518958682 @default.
• W2109241881 cites W1535342352 @default.
• W2109241881 cites W1557544665 @default.
• W2109241881 cites W1967756702 @default.
• W2109241881 cites W1974140457 @default.
• W2109241881 cites W1991887798 @default.
• W2109241881 cites W1997403299 @default.
• W2109241881 cites W2008071343 @default.
• W2109241881 cites W2009220320 @default.
• W2109241881 cites W2023234589 @default.
• W2109241881 cites W2042995004 @default.
• W2109241881 cites W2044275765 @default.
• W2109241881 cites W2044296969 @default.
• W2109241881 cites W2055759808 @default.
• W2109241881 cites W2086752646 @default.
• W2109241881 cites W2098264302 @default.
• W2109241881 cites W2184063559 @default.
• W2109241881 doi "https://doi.org/10.1002/(sici)1097-0290(19980905)59:5<567::aid-bit6>3.0.co;2-8" @default.
• W2109241881 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10099373" @default.
• W2109241881 hasPublicationYear "1998" @default.
• W2109241881 type Work @default.
• W2109241881 sameAs 2109241881 @default.
• W2109241881 citedByCount "124" @default.
• W2109241881 countsByYear W21092418812012 @default.
• W2109241881 countsByYear W21092418812013 @default.
• W2109241881 countsByYear W21092418812014 @default.
• W2109241881 countsByYear W21092418812015 @default.
• W2109241881 countsByYear W21092418812016 @default.
• W2109241881 countsByYear W21092418812017 @default.
• W2109241881 countsByYear W21092418812018 @default.
• W2109241881 countsByYear W21092418812019 @default.
• W2109241881 countsByYear W21092418812020 @default.
• W2109241881 countsByYear W21092418812021 @default.
• W2109241881 countsByYear W21092418812022 @default.
• W2109241881 countsByYear W21092418812023 @default.
• W2109241881 crossrefType "journal-article" @default.
• W2109241881 hasAuthorship W2109241881A5004289043 @default.
• W2109241881 hasAuthorship W2109241881A5013737653 @default.
• W2109241881 hasAuthorship W2109241881A5069297606 @default.
• W2109241881 hasAuthorship W2109241881A5079295525 @default.
• W2109241881 hasConcept C104317684 @default.
• W2109241881 hasConcept C121050878 @default.
• W2109241881 hasConcept C153911025 @default.
• W2109241881 hasConcept C168170006 @default.
• W2109241881 hasConcept C170344550 @default.
• W2109241881 hasConcept C199360897 @default.
• W2109241881 hasConcept C32470452 @default.
• W2109241881 hasConcept C40767141 @default.
• W2109241881 hasConcept C41008148 @default.
• W2109241881 hasConcept C54355233 @default.
• W2109241881 hasConcept C55493867 @default.
• W2109241881 hasConcept C59822182 @default.
• W2109241881 hasConcept C81089528 @default.
• W2109241881 hasConcept C81885089 @default.
• W2109241881 hasConcept C86803240 @default.
• W2109241881 hasConceptScore W2109241881C104317684 @default.
• W2109241881 hasConceptScore W2109241881C121050878 @default.
• W2109241881 hasConceptScore W2109241881C153911025 @default.
• W2109241881 hasConceptScore W2109241881C168170006 @default.
• W2109241881 hasConceptScore W2109241881C170344550 @default.
• W2109241881 hasConceptScore W2109241881C199360897 @default.
• W2109241881 hasConceptScore W2109241881C32470452 @default.
• W2109241881 hasConceptScore W2109241881C40767141 @default.
• W2109241881 hasConceptScore W2109241881C41008148 @default.
• W2109241881 hasConceptScore W2109241881C54355233 @default.
• W2109241881 hasConceptScore W2109241881C55493867 @default.
• W2109241881 hasConceptScore W2109241881C59822182 @default.
• W2109241881 hasConceptScore W2109241881C81089528 @default.
• W2109241881 hasConceptScore W2109241881C81885089 @default.
• W2109241881 hasConceptScore W2109241881C86803240 @default.
• W2109241881 hasIssue "5" @default.
• W2109241881 hasLocation W21092418811 @default.
• W2109241881 hasLocation W21092418812 @default.
• W2109241881 hasOpenAccess W2109241881 @default.
• W2109241881 hasPrimaryLocation W21092418811 @default.
• W2109241881 hasRelatedWork W1987653914 @default.
• W2109241881 hasRelatedWork W2040528063 @default.
• W2109241881 hasRelatedWork W2046308467 @default.
• W2109241881 hasRelatedWork W2082762559 @default.
• W2109241881 hasRelatedWork W2376673900 @default.
• W2109241881 hasRelatedWork W2377327490 @default.
• W2109241881 hasRelatedWork W2410849822 @default.
• W2109241881 hasRelatedWork W3008791973 @default.
• W2109241881 hasRelatedWork W2560253012 @default.
• W2109241881 hasRelatedWork W2750811721 @default.
• W2109241881 hasVolume "59" @default.
• W2109241881 isParatext "false" @default.
• W2109241881 isRetracted "false" @default.
• W2109241881 magId "2109241881" @default.

• next