Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109368575> ?p ?o ?g. }
- W2109368575 endingPage "29" @default.
- W2109368575 startingPage "1" @default.
- W2109368575 abstract "Glucosylceramide (GlcCer) accumulates in the inherited metabolic disorder, Gaucher disease, because of the defective activity of lysosomal glucocerebrosidase. We previously demonstrated that upon GlcCer accumulation, cultured hippocampal neurons exhibit modified growth patterns, altered endoplasmic reticulum density, and altered calcium release from intracellular stores. We here examined the relationship between GlcCer accumulation and phospholipid synthesis. After treatment of neurons with an active site-directed inhibitor of glucocerebrosidase, or in neurons obtained from a mouse model of Gaucher disease, [14C]methyl choline incorporation into [14C]phosphatidylcholine ([14C]PC) and [14C]sphingomyelin was elevated, as were [14C]CDP-choline levels, suggesting that CTP:phosphocholine cytidylyltransferase (CCT) is activated. Indeed, CCT activity was elevated in neurons that had accumulated GlcCer. GlcCer, but not galactosylceramide (GalCer), stimulated CCT activity in rat brain homogenates, and significantly higher levels of CCT were membrane associated in cortical homogenates from a mouse model of Gaucher disease compared with wild-type mice. Because CCT mRNA and protein levels were unaltered in either neurons or brain tissue that had accumulated GlcCer, it appeared likely that GlcCer activates CCT by a post-translational mechanism. This was verified by examination of the effect of GlcCer on CCT purified about 1200-fold from rat brain. GlcCer stimulated CCT activity, with stimulation observed at levels as low as 2.5 mol% and with maximal activation reached at 10 mol%. In contrast, GalCer had no effect. Together, these data demonstrate that GlcCer directly activates CCT, which results in elevated PC synthesis, which may account for some of the changes in growth rates observed upon neuronal GlcCer accumulation." @default.
- W2109368575 created "2016-06-24" @default.
- W2109368575 creator A5024228703 @default.
- W2109368575 creator A5055850736 @default.
- W2109368575 creator A5067203212 @default.
- W2109368575 creator A5068907124 @default.
- W2109368575 creator A5085526348 @default.
- W2109368575 date "2002-09-05" @default.
- W2109368575 modified "2023-10-18" @default.
- W2109368575 title "Phosphatidylcholine synthesis is elevated in neuronal models of Gaucher disease due to direct activation of CTP:phosphocholine cytidylyltransferase by glucosylceramide" @default.
- W2109368575 cites W1368384 @default.
- W2109368575 cites W1522819451 @default.
- W2109368575 cites W1524917676 @default.
- W2109368575 cites W1526562091 @default.
- W2109368575 cites W1527487900 @default.
- W2109368575 cites W1535040964 @default.
- W2109368575 cites W1560906910 @default.
- W2109368575 cites W1576342917 @default.
- W2109368575 cites W1594700564 @default.
- W2109368575 cites W1598399972 @default.
- W2109368575 cites W1620047152 @default.
- W2109368575 cites W162053295 @default.
- W2109368575 cites W1767656624 @default.
- W2109368575 cites W1777151528 @default.
- W2109368575 cites W1815261168 @default.
- W2109368575 cites W1909266718 @default.
- W2109368575 cites W1969062984 @default.
- W2109368575 cites W1970602831 @default.
- W2109368575 cites W1971392424 @default.
- W2109368575 cites W1977779461 @default.
- W2109368575 cites W1983571376 @default.
- W2109368575 cites W1991321700 @default.
- W2109368575 cites W1994758211 @default.
- W2109368575 cites W1996965660 @default.
- W2109368575 cites W1999919910 @default.
- W2109368575 cites W2003525182 @default.
- W2109368575 cites W2006376420 @default.
- W2109368575 cites W2009896071 @default.
- W2109368575 cites W2010749115 @default.
- W2109368575 cites W2011556695 @default.
- W2109368575 cites W2020673988 @default.
- W2109368575 cites W2022024194 @default.
- W2109368575 cites W2023378452 @default.
- W2109368575 cites W2024493352 @default.
- W2109368575 cites W2039029470 @default.
- W2109368575 cites W2042512150 @default.
- W2109368575 cites W2044087089 @default.
- W2109368575 cites W2045424066 @default.
- W2109368575 cites W2045466436 @default.
- W2109368575 cites W2047196688 @default.
- W2109368575 cites W2052620101 @default.
- W2109368575 cites W2055445325 @default.
- W2109368575 cites W2058341443 @default.
- W2109368575 cites W2058811179 @default.
- W2109368575 cites W2061213728 @default.
- W2109368575 cites W2068015921 @default.
- W2109368575 cites W2074300163 @default.
- W2109368575 cites W2080952606 @default.
- W2109368575 cites W2085186268 @default.
- W2109368575 cites W2090439479 @default.
- W2109368575 cites W2099218936 @default.
- W2109368575 cites W2100837269 @default.
- W2109368575 cites W2102100103 @default.
- W2109368575 cites W2124717330 @default.
- W2109368575 cites W2141810718 @default.
- W2109368575 cites W2168526937 @default.
- W2109368575 cites W2181534440 @default.
- W2109368575 cites W2264865153 @default.
- W2109368575 cites W2401738727 @default.
- W2109368575 cites W2419095632 @default.
- W2109368575 cites W276004829 @default.
- W2109368575 cites W4293247451 @default.
- W2109368575 cites W4313152550 @default.
- W2109368575 doi "https://doi.org/10.1096/fj.02-0149fje" @default.
- W2109368575 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12223447" @default.
- W2109368575 hasPublicationYear "2002" @default.
- W2109368575 type Work @default.
- W2109368575 sameAs 2109368575 @default.
- W2109368575 citedByCount "73" @default.
- W2109368575 countsByYear W21093685752012 @default.
- W2109368575 countsByYear W21093685752013 @default.
- W2109368575 countsByYear W21093685752014 @default.
- W2109368575 countsByYear W21093685752015 @default.
- W2109368575 countsByYear W21093685752016 @default.
- W2109368575 countsByYear W21093685752017 @default.
- W2109368575 countsByYear W21093685752018 @default.
- W2109368575 countsByYear W21093685752019 @default.
- W2109368575 countsByYear W21093685752020 @default.
- W2109368575 countsByYear W21093685752021 @default.
- W2109368575 countsByYear W21093685752022 @default.
- W2109368575 countsByYear W21093685752023 @default.
- W2109368575 crossrefType "journal-article" @default.
- W2109368575 hasAuthorship W2109368575A5024228703 @default.
- W2109368575 hasAuthorship W2109368575A5055850736 @default.
- W2109368575 hasAuthorship W2109368575A5067203212 @default.
- W2109368575 hasAuthorship W2109368575A5068907124 @default.
- W2109368575 hasAuthorship W2109368575A5085526348 @default.
- W2109368575 hasConcept C126322002 @default.