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• W2109390205 abstract "Microautoradiography (MAR) is a conventional imaging method based on the daguerreotype. The technique is used to visualize the distribution of radionuclide-labeled compounds within a tissue section. However, application of the classical MAR method to plant tissue sections is associated with several difficulties. In this study, we report an MAR method applicable to fresh-frozen plant sections. Our method had two features: (i) the sample was kept frozen from plant tissue collection to radioisotope detection, making it possible to fix solutes without solvent exchange; and (ii) 1.2 µm thick polyphenylene sulfide film was inserted between the fresh-frozen plant section and the photosensitive nuclear emulsion to separate the section from the emulsion before autoradiography was conducted, which significantly improved the quality of the section until microscopic detection, the quality of the MAR image and the success rate. Then, the passage of cadmium (Cd) through vegetative rice stem tissue after 24 h of 109Cd absorption was described for the first time using the MAR method. MAR clearly revealed the distribution of 109Cd at the tissue level with high resolution. The 109Cd concentration in phloem cells was found to be particularly high, whereas the xylem cells contained only small amounts of 109Cd. The MAR method was also applicable for detecting 109Cd and [33P]phosphate in roots. The MAR method developed here is expected to provide distribution images for a variety of compounds and ions in plant tissue." @default.
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• W2109390205 date "2014-04-18" @default.
• W2109390205 modified "2023-10-01" @default.
• W2109390205 title "A Microautoradiographic Method for Fresh-Frozen Sections to Reveal the Distribution of Radionuclides at the Cellular Level in Plants" @default.
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• W2109390205 doi "https://doi.org/10.1093/pcp/pcu056" @default.
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