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- W2109414551 abstract "Abstract Schizosaccharomyces pombe Rhp55 and Rhp57 are RecA-like proteins involved in double-strand break (DSB) repair. Here we demonstrate that Rhp55 and Rhp57 proteins strongly interact in vivo, similar to Saccharomyces cerevisiae Rad55p and Rad57p. Mutations in the conserved ATP-binding/hydrolysis folds of both the Rhp55 and Rhp57 proteins impaired their function in DNA repair but not in cell proliferation. However, when combined, ATPase fold mutations in Rhp55p and Rhp57p resulted in severe defects of both functions, characteristic of the deletion mutants. Yeast two-hybrid analysis also revealed other multiple in vivo interactions among S. pombe proteins involved in recombinational DNA repair. Similar to S. cerevisiae Rad51p-Rad54p, S. pombe Rhp51p and Rhp54p were found to interact. Both putative Rad52 homologs in S. pombe, Rad22p and Rti1p, were found to interact with the C-terminal region of Rhp51 protein. Moreover, Rad22p and Rti1p exhibited mutual, as well as self-, interactions. In contrast to the S. cerevisiae interacting pair Rad51p-Rad55p, S. pombe Rhp51 protein strongly interacted with Rhp57 but not with Rhp55 protein. In addition, the Rti1 and Rad22 proteins were found to form a complex with the large subunit of S. pombe RPA. Our data provide compelling evidence that most, but not all, of the protein-protein interactions found in S. cerevisiae DSB repair are evolutionarily conserved." @default.
- W2109414551 created "2016-06-24" @default.
- W2109414551 creator A5029305897 @default.
- W2109414551 creator A5053691948 @default.
- W2109414551 creator A5054343829 @default.
- W2109414551 creator A5062214131 @default.
- W2109414551 creator A5089708101 @default.
- W2109414551 date "2001-09-01" @default.
- W2109414551 modified "2023-10-06" @default.
- W2109414551 title "Multiple Interactions Among the Components of the Recombinational DNA Repair System in <i>Schizosaccharomyces pombe</i>" @default.
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- W2109414551 doi "https://doi.org/10.1093/genetics/159.1.91" @default.
- W2109414551 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1461803" @default.
- W2109414551 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11560889" @default.
- W2109414551 hasPublicationYear "2001" @default.
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