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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109437083> ?p ?o ?g. }

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• W2109437083 abstract "In skeletal muscles, the expression of neuronal NO synthase (nNOS) isoforms is uncharacterized at the protein level. We therefore conducted epitope mapping with anti-peptide-antibodies. Antibodies specific for the nNOS N-terminus recognized the 160-kDa alpha-isoform. In contrast, antibodies against the middle portion or the C-terminus of nNOS bound additionally to the truncated 140-kDa beta-isoform which lacks the PDZ-domain present in the alpha-isoform. All nNOS immunohistochemical reactivity was confined to the sarcolemma. Consistently, immunoblotting disclosed both nNOS-isoforms to be co-enriched in the membrane-associated fractions. The beta-isoform was co-immunoprecipitated with alpha-isoform antibodies in muscle extracts indicating an association of both nNOS-isoforms to direct the beta-variant to the sarcolemma." @default.
• W2109437083 created "2016-06-24" @default.
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• W2109437083 date "2011-09-19" @default.
• W2109437083 modified "2023-10-16" @default.
• W2109437083 title "The beta-isoform of neuronal nitric oxide synthase (nNOS) lacking the PDZ domain is localized at the sarcolemma" @default.
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• W2109437083 doi "https://doi.org/10.1016/j.febslet.2011.09.016" @default.
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