Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109458220> ?p ?o ?g. }
- W2109458220 endingPage "585" @default.
- W2109458220 startingPage "574" @default.
- W2109458220 abstract "Abstract IMMUTANS (IM) encodes a thylakoid membrane protein that has been hypothesized to act as a terminal oxidase that couples the reduction of O2 to the oxidation of the plastoquinone (PQ) pool of the photosynthetic electron transport chain. Because IM shares sequence similarity to the stress-induced mitochondrial alternative oxidase (AOX), it has been suggested that the protein encoded by IM acts as a safety valve during the generation of excess photosynthetically generated electrons. We combined in vivo chlorophyll fluorescence quenching analyses with measurements of the redox state of P 700 to assess the capacity of IM to compete with photosystem I for intersystem electrons during steady-state photosynthesis in Arabidopsis (Arabidopsis thaliana). Comparisons were made between wild-type plants, im mutant plants, as well as transgenics in which IM protein levels had been overexpressed six (OE-6×) and 16 (OE-16×) times. Immunoblots indicated that IM abundance was the only major variant that we could detect between these genotypes. Overexpression of IM did not result in increased capacity to keep the PQ pool oxidized compared to either the wild type or im grown under control conditions (25°C and photosynthetic photon flux density of 150 μmol photons m−2 s−1). Similar results were observed either after 3-d cold stress at 5°C or after full-leaf expansion at 5°C and photosynthetic photon flux density of 150 μmol photons m−2 s−1. Furthermore, IM abundance did not enhance protection of either photosystem II or photosystem I from photoinhibition at either 25°C or 5°C. Our in vivo data indicate that modulation of IM expression and polypeptide accumulation does not alter the flux of intersystem electrons to P 700 + during steady-state photosynthesis and does not provide any significant photoprotection. In contrast to AOX1a, meta-analyses of published Arabidopsis microarray data indicated that IM expression exhibited minimal modulation in response to myriad abiotic stresses, which is consistent with our functional data. However, IM exhibited significant modulation in response to development in concert with changes in AOX1a expression. Thus, neither our functional analyses of the IM knockout and overexpression lines nor meta-analyses of gene expression support the model that IM acts as a safety valve to regulate the redox state of the PQ pool during stress and acclimation. Rather, IM appears to be strongly regulated by developmental stage of Arabidopsis." @default.
- W2109458220 created "2016-06-24" @default.
- W2109458220 creator A5006204902 @default.
- W2109458220 creator A5016448961 @default.
- W2109458220 creator A5019244369 @default.
- W2109458220 creator A5031339189 @default.
- W2109458220 creator A5041388117 @default.
- W2109458220 creator A5046590788 @default.
- W2109458220 creator A5046832101 @default.
- W2109458220 creator A5054409475 @default.
- W2109458220 creator A5079716497 @default.
- W2109458220 creator A5080342124 @default.
- W2109458220 creator A5089294038 @default.
- W2109458220 creator A5090653729 @default.
- W2109458220 date "2006-08-04" @default.
- W2109458220 modified "2023-10-14" @default.
- W2109458220 title "IMMUTANS Does Not Act as a Stress-Induced Safety Valve in the Protection of the Photosynthetic Apparatus of Arabidopsis during Steady-State Photosynthesis" @default.
- W2109458220 cites W1269979427 @default.
- W2109458220 cites W153105862 @default.
- W2109458220 cites W1604364822 @default.
- W2109458220 cites W1963908376 @default.
- W2109458220 cites W1965381818 @default.
- W2109458220 cites W1965698143 @default.
- W2109458220 cites W1973050962 @default.
- W2109458220 cites W1978636793 @default.
- W2109458220 cites W1982775480 @default.
- W2109458220 cites W1983729367 @default.
- W2109458220 cites W1987529977 @default.
- W2109458220 cites W1988795266 @default.
- W2109458220 cites W1996645811 @default.
- W2109458220 cites W2003748109 @default.
- W2109458220 cites W2005270779 @default.
- W2109458220 cites W2005501787 @default.
- W2109458220 cites W2005885010 @default.
- W2109458220 cites W2012343942 @default.
- W2109458220 cites W2018289835 @default.
- W2109458220 cites W2023831001 @default.
- W2109458220 cites W2028686058 @default.
- W2109458220 cites W2032723598 @default.
- W2109458220 cites W2037853364 @default.
- W2109458220 cites W2038161366 @default.
- W2109458220 cites W2044242703 @default.
- W2109458220 cites W2044280701 @default.
- W2109458220 cites W2046651058 @default.
- W2109458220 cites W2051230539 @default.
- W2109458220 cites W2053427298 @default.
- W2109458220 cites W2056425137 @default.
- W2109458220 cites W2059530505 @default.
- W2109458220 cites W2060159166 @default.
- W2109458220 cites W2062634763 @default.
- W2109458220 cites W2064383636 @default.
- W2109458220 cites W2065717544 @default.
- W2109458220 cites W2067748791 @default.
- W2109458220 cites W2068405599 @default.
- W2109458220 cites W2073317899 @default.
- W2109458220 cites W2073597759 @default.
- W2109458220 cites W2076151827 @default.
- W2109458220 cites W2085812602 @default.
- W2109458220 cites W2089692553 @default.
- W2109458220 cites W2100409610 @default.
- W2109458220 cites W2100837269 @default.
- W2109458220 cites W2104237015 @default.
- W2109458220 cites W2105796315 @default.
- W2109458220 cites W2107491970 @default.
- W2109458220 cites W2109324504 @default.
- W2109458220 cites W2111346528 @default.
- W2109458220 cites W2111826292 @default.
- W2109458220 cites W2116122104 @default.
- W2109458220 cites W2123150282 @default.
- W2109458220 cites W2127974864 @default.
- W2109458220 cites W2132541664 @default.
- W2109458220 cites W2135112360 @default.
- W2109458220 cites W2138676381 @default.
- W2109458220 cites W2146405286 @default.
- W2109458220 cites W2153318923 @default.
- W2109458220 cites W2157330318 @default.
- W2109458220 cites W2157742117 @default.
- W2109458220 cites W2158499051 @default.
- W2109458220 cites W2162946468 @default.
- W2109458220 cites W2163803810 @default.
- W2109458220 cites W2166412331 @default.
- W2109458220 cites W2179769898 @default.
- W2109458220 cites W2193189274 @default.
- W2109458220 cites W2289046777 @default.
- W2109458220 cites W2318877471 @default.
- W2109458220 cites W4242470225 @default.
- W2109458220 doi "https://doi.org/10.1104/pp.106.085886" @default.
- W2109458220 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1586030" @default.
- W2109458220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16891546" @default.
- W2109458220 hasPublicationYear "2006" @default.
- W2109458220 type Work @default.
- W2109458220 sameAs 2109458220 @default.
- W2109458220 citedByCount "109" @default.
- W2109458220 countsByYear W21094582202012 @default.
- W2109458220 countsByYear W21094582202013 @default.
- W2109458220 countsByYear W21094582202014 @default.
- W2109458220 countsByYear W21094582202015 @default.
- W2109458220 countsByYear W21094582202016 @default.