FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109474075> ?p ?o ?g. }

• W2109474075 endingPage "311" @default.
• W2109474075 startingPage "301" @default.
• W2109474075 abstract "We probed the complex between the replication origin, oriC, and the initiator protein DnaA using different types of mutations in the five binding sites for DnaA, DnaA boxes R1-R4 and M: (i) point mutations in individual DnaA boxes and combinations of them; (ii) replacement of the DnaA boxes by a scrambled 9 bp non-box motif; (iii) positional exchange; and (iv) inversion of the DnaA boxes. For each of the five DnaA boxes we found at least one type of mutation that resulted in a phenotype. This demonstrates that all DnaA boxes in oriC have a function in the initiation process. Most mutants with point mutations retained some origin activity, and the in vitro DnaA-binding capacity of these origins correlated well with their replication proficiency. Inversion or scrambling of DnaA boxes R1 or M inactivated oriC-dependent replication of joint replicons or minichromosomes under all conditions, demonstrating the importance of these sites. In contrast, mutants with inverted or scrambled DnaA boxes R2 or R4 could not replicate in wild-type hosts but gave transformants in host strains with deleted or compromised chromosomal oriC at elevated DnaA concentrations. We conclude that these origins require more DnaA per origin for initiation than does wild-type oriC. Mutants in DnaA box R3 behaved essentially like wild-type oriC, except for those in which the low-affinity box R3 was replaced by the high-affinity box R1. Apparently, initiation is possible without DnaA binding to box R3, but high-affinity DnaA binding to DnaA box R3 upsets the regulation. Taken together, these results demonstrate that there are finely tuned DnaA binding requirements for each of the individual DnaA boxes for optimal build-up of the initiation complex and replication initiation in vivo." @default.
• W2109474075 created "2016-06-24" @default.
• W2109474075 creator A5021340844 @default.
• W2109474075 creator A5031149081 @default.
• W2109474075 creator A5059634856 @default.
• W2109474075 creator A5060551158 @default.
• W2109474075 creator A5078528804 @default.
• W2109474075 date "1996-07-01" @default.
• W2109474075 modified "2023-10-06" @default.
• W2109474075 title "<b>A comprehensive set of DnaA‐box mutations in the replication origin, <i>oriC</i>, of <i>Escherichia coli</i></b>" @default.
• W2109474075 cites W13671887 @default.
• W2109474075 cites W1530522271 @default.
• W2109474075 cites W1587598493 @default.
• W2109474075 cites W1591715670 @default.
• W2109474075 cites W1603041127 @default.
• W2109474075 cites W1605117055 @default.
• W2109474075 cites W1616137642 @default.
• W2109474075 cites W1634613156 @default.
• W2109474075 cites W189144760 @default.
• W2109474075 cites W1988260266 @default.
• W2109474075 cites W1994078496 @default.
• W2109474075 cites W1998462937 @default.
• W2109474075 cites W1999752033 @default.
• W2109474075 cites W1999859839 @default.
• W2109474075 cites W2018060326 @default.
• W2109474075 cites W2019081472 @default.
• W2109474075 cites W2020183116 @default.
• W2109474075 cites W2023758956 @default.
• W2109474075 cites W2024288577 @default.
• W2109474075 cites W2035100615 @default.
• W2109474075 cites W2035486490 @default.
• W2109474075 cites W2036931150 @default.
• W2109474075 cites W2050191390 @default.
• W2109474075 cites W2054243030 @default.
• W2109474075 cites W2058322547 @default.
• W2109474075 cites W2061812908 @default.
• W2109474075 cites W2068518785 @default.
• W2109474075 cites W2070469209 @default.
• W2109474075 cites W2072874055 @default.
• W2109474075 cites W207515165 @default.
• W2109474075 cites W2075784524 @default.
• W2109474075 cites W2079272484 @default.
• W2109474075 cites W2083874809 @default.
• W2109474075 cites W2084874442 @default.
• W2109474075 cites W2086891026 @default.
• W2109474075 cites W2098255930 @default.
• W2109474075 cites W2144634347 @default.
• W2109474075 cites W2145867940 @default.
• W2109474075 cites W2161500975 @default.
• W2109474075 cites W21910474 @default.
• W2109474075 cites W223306453 @default.
• W2109474075 cites W2502974091 @default.
• W2109474075 cites W285557247 @default.
• W2109474075 cites W3150699962 @default.
• W2109474075 cites W80165809 @default.
• W2109474075 doi "https://doi.org/10.1046/j.1365-2958.1996.6481362.x" @default.
• W2109474075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8858585" @default.
• W2109474075 hasPublicationYear "1996" @default.
• W2109474075 type Work @default.
• W2109474075 sameAs 2109474075 @default.
• W2109474075 citedByCount "75" @default.
• W2109474075 countsByYear W21094740752013 @default.
• W2109474075 countsByYear W21094740752014 @default.
• W2109474075 countsByYear W21094740752015 @default.
• W2109474075 countsByYear W21094740752016 @default.
• W2109474075 countsByYear W21094740752017 @default.
• W2109474075 countsByYear W21094740752018 @default.
• W2109474075 countsByYear W21094740752019 @default.
• W2109474075 countsByYear W21094740752021 @default.
• W2109474075 countsByYear W21094740752023 @default.
• W2109474075 crossrefType "journal-article" @default.
• W2109474075 hasAuthorship W2109474075A5021340844 @default.
• W2109474075 hasAuthorship W2109474075A5031149081 @default.
• W2109474075 hasAuthorship W2109474075A5059634856 @default.
• W2109474075 hasAuthorship W2109474075A5060551158 @default.
• W2109474075 hasAuthorship W2109474075A5078528804 @default.
• W2109474075 hasConcept C104317684 @default.
• W2109474075 hasConcept C107827726 @default.
• W2109474075 hasConcept C143065580 @default.
• W2109474075 hasConcept C153911025 @default.
• W2109474075 hasConcept C176944494 @default.
• W2109474075 hasConcept C54355233 @default.
• W2109474075 hasConcept C552990157 @default.
• W2109474075 hasConcept C58337764 @default.
• W2109474075 hasConcept C73573662 @default.
• W2109474075 hasConcept C86339819 @default.
• W2109474075 hasConcept C86803240 @default.
• W2109474075 hasConcept C94966510 @default.
• W2109474075 hasConceptScore W2109474075C104317684 @default.
• W2109474075 hasConceptScore W2109474075C107827726 @default.
• W2109474075 hasConceptScore W2109474075C143065580 @default.
• W2109474075 hasConceptScore W2109474075C153911025 @default.
• W2109474075 hasConceptScore W2109474075C176944494 @default.
• W2109474075 hasConceptScore W2109474075C54355233 @default.
• W2109474075 hasConceptScore W2109474075C552990157 @default.
• W2109474075 hasConceptScore W2109474075C58337764 @default.
• W2109474075 hasConceptScore W2109474075C73573662 @default.
• W2109474075 hasConceptScore W2109474075C86339819 @default.

• next