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• W2109489904 abstract "Abstract Cefprozil (CPZ) is a second‐generation semi‐synthetic cephalosporin antibiotic that commonly exists as the mixture of Z and E diastereoisomers, at the ratio of approximately 9:1. A novel reversed‐phase HPLC method for the determination of CPZ in tablets was described. The separation of CPZ diastereoisomers and caffeine (internal standard) was carried out by applying the same analytical and instrumental conditions on two stationary phases, which have different surface chemistries. The columns used in the study were monolithic silica Merck Chromolith Performance RP‐18e and conventional C 18 silica Phenomenex Synergi Hydro RP columns. In total, 10 μL aliquots of samples were injected into the system and eluted using water–acetonitrile (90:10, v/v) solution, which was pumped through the column at a flow rate of 1.0 mL/min. The analyte peaks were detected at 200 nm using diode array detector with high specificity. CPZ diastereoisomers and caffeine were measured within 13 min using the C 18 column, whereas <5 min was required for the monolithic one. Validation studies were performed according to official recommendations. Value of a monolithic column for the assay of diastereoisomers in pharmaceutical tablets was evaluated for the first time and found as a powerful alternative to highly efficient C 18 columns." @default.
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• W2109489904 date "2011-05-06" @default.
• W2109489904 modified "2023-10-16" @default.
• W2109489904 title "HPLC determination of cefprozil in tablets using monolithic and C₁₈ silica columns" @default.
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• W2109489904 doi "https://doi.org/10.1002/jssc.201100170" @default.
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