Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109497034> ?p ?o ?g. }
- W2109497034 endingPage "3304" @default.
- W2109497034 startingPage "3289" @default.
- W2109497034 abstract "Saccharomyces cerevisiae Pkh1 and Pkh2 (orthologues of mammalian protein kinase, PDK1) are functionally redundant. These kinases activate three AGC family kinases involved in the maintenance of cell wall integrity: Ypk1 and Ypk2, two closely related, functionally redundant enzymes (orthologues of mammalian protein kinase SGK), and Pkc1 (orthologue of mammalian protein kinase PRK2). Pkh1 and Pkh2 activate Ypk1, Ypk2 and Pkc1 by phosphorylating a Thr in a conserved sequence motif (PDK1 site) within the activation loop of these proteins. A fourth protein kinase involved in growth control and stress response, Sch9 (orthologue of mammalian protein kinase c-Akt/PKB), also carries the conserved activation loop motif. Like other AGC family kinases, Ypk1, Ypk2, Pkc1 and Sch9 also carry a second conserved sequence motif situated in a region C-terminal to the catalytic domain, called the hydrophobic motif (PDK2 site). Currently, there is still controversy surrounding the identity of the enzyme responsible for phosphorylating this second site and the necessity for phosphorylation at this site for in vivo function. Here, genetic and biochemical methods have been used to investigate the physiological consequences of phosphorylation at the PDK1 and PDK2 sites of Ypk1, Pkc1 and Sch9. It was found that phosphorylation at the PDK1 site in the activation loop is indispensable for the essential functions of all three kinases in vivo, whereas phosphorylation at the PDK2 motif plays a non-essential and much more subtle role in modulating the ability of these kinases to regulate the downstream processes in which they participate." @default.
- W2109497034 created "2016-06-24" @default.
- W2109497034 creator A5048920959 @default.
- W2109497034 creator A5062643532 @default.
- W2109497034 creator A5072948179 @default.
- W2109497034 date "2004-10-01" @default.
- W2109497034 modified "2023-09-23" @default.
- W2109497034 title "Differential roles of PDK1- and PDK2-phosphorylation sites in the yeast AGC kinases Ypk1, Pkc1 and Sch9" @default.
- W2109497034 cites W104690111 @default.
- W2109497034 cites W111392919 @default.
- W2109497034 cites W1608303629 @default.
- W2109497034 cites W163818535 @default.
- W2109497034 cites W1726025741 @default.
- W2109497034 cites W1826524642 @default.
- W2109497034 cites W1864334043 @default.
- W2109497034 cites W1908445283 @default.
- W2109497034 cites W1964716929 @default.
- W2109497034 cites W1964881184 @default.
- W2109497034 cites W1965976637 @default.
- W2109497034 cites W1970548320 @default.
- W2109497034 cites W1972718290 @default.
- W2109497034 cites W1976764418 @default.
- W2109497034 cites W1978286648 @default.
- W2109497034 cites W1984736455 @default.
- W2109497034 cites W1990332775 @default.
- W2109497034 cites W1990841830 @default.
- W2109497034 cites W1991468122 @default.
- W2109497034 cites W1992069812 @default.
- W2109497034 cites W1992474764 @default.
- W2109497034 cites W1992718750 @default.
- W2109497034 cites W1997447529 @default.
- W2109497034 cites W1999163644 @default.
- W2109497034 cites W2002622198 @default.
- W2109497034 cites W2003103378 @default.
- W2109497034 cites W2004926623 @default.
- W2109497034 cites W2008943739 @default.
- W2109497034 cites W2010422684 @default.
- W2109497034 cites W2012142261 @default.
- W2109497034 cites W2012880207 @default.
- W2109497034 cites W2017370430 @default.
- W2109497034 cites W2017882961 @default.
- W2109497034 cites W2018380860 @default.
- W2109497034 cites W2018981756 @default.
- W2109497034 cites W2021788902 @default.
- W2109497034 cites W2023012828 @default.
- W2109497034 cites W2023472659 @default.
- W2109497034 cites W2029787353 @default.
- W2109497034 cites W2030720692 @default.
- W2109497034 cites W2030774127 @default.
- W2109497034 cites W2033499305 @default.
- W2109497034 cites W2039193709 @default.
- W2109497034 cites W2041572922 @default.
- W2109497034 cites W2041926656 @default.
- W2109497034 cites W2046491160 @default.
- W2109497034 cites W2046525768 @default.
- W2109497034 cites W2051440750 @default.
- W2109497034 cites W2052550515 @default.
- W2109497034 cites W2053151896 @default.
- W2109497034 cites W2058138221 @default.
- W2109497034 cites W2061171666 @default.
- W2109497034 cites W2063852327 @default.
- W2109497034 cites W2066618807 @default.
- W2109497034 cites W2068432705 @default.
- W2109497034 cites W2068766832 @default.
- W2109497034 cites W2075725057 @default.
- W2109497034 cites W2079100882 @default.
- W2109497034 cites W2079123009 @default.
- W2109497034 cites W2079741758 @default.
- W2109497034 cites W2088127501 @default.
- W2109497034 cites W2088574064 @default.
- W2109497034 cites W2091275711 @default.
- W2109497034 cites W2091512138 @default.
- W2109497034 cites W2100837269 @default.
- W2109497034 cites W2101108802 @default.
- W2109497034 cites W2103002807 @default.
- W2109497034 cites W2103427562 @default.
- W2109497034 cites W2103941658 @default.
- W2109497034 cites W2105085077 @default.
- W2109497034 cites W2115073295 @default.
- W2109497034 cites W2122005428 @default.
- W2109497034 cites W2128635872 @default.
- W2109497034 cites W2129742695 @default.
- W2109497034 cites W2132417094 @default.
- W2109497034 cites W2138270253 @default.
- W2109497034 cites W2146157811 @default.
- W2109497034 cites W2146989813 @default.
- W2109497034 cites W2147034765 @default.
- W2109497034 cites W2147398227 @default.
- W2109497034 cites W2152600551 @default.
- W2109497034 cites W2158745573 @default.
- W2109497034 cites W2162193671 @default.
- W2109497034 cites W2165710306 @default.
- W2109497034 cites W2169333620 @default.
- W2109497034 cites W2169494292 @default.
- W2109497034 cites W2170633329 @default.
- W2109497034 cites W27250448 @default.
- W2109497034 doi "https://doi.org/10.1099/mic.0.27286-0" @default.
- W2109497034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15470109" @default.