FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109556475> ?p ?o ?g. }

• W2109556475 endingPage "609" @default.
• W2109556475 startingPage "596" @default.
• W2109556475 abstract "Aliphatic amidases (EC 3.5.1.4) are enzymes catalysing the hydrolysis of short-chain amides to produce ammonia and the corresponding organic acid. Such an amidase, AmiE, has been detected previously in Helicobacter pylori. Analysis of the complete H. pylori genome sequence revealed the existence of a duplicated amidase gene that we named amiF. The corresponding AmiF protein is 34% identical to its AmiE paralogue. Because gene duplication is widely considered to be a fundamental process in the acquisition of novel enzymatic functions, we decided to study and compare the functions of the paralogous amidases of H. pylori. AmiE and AmiF proteins were overproduced in Escherichia coli and purified by a two-step chromatographic procedure. The two H. pylori amidases could be distinguished by different biochemical characteristics such as optimum pH or temperature. AmiE hydrolysed propionamide, acetamide and acrylamide and had no activity with formamide. AmiF presented an unexpected substrate specificity: it only hydrolysed formamide. AmiF is thus the first formamidase (EC 3.5.1.49) related to aliphatic amidases to be described. Cys-165 in AmiE and Cys-166 in AmiF were identified as residues essential for catalysis of the corresponding enzymes. H. pylori strains carrying single and double mutations of amiE and amiF were constructed. The substrate specificities of these enzymes were confirmed in H. pylori. Production of AmiE and AmiF proteins is dependent on the activity of other enzymes involved in the nitrogen metabolism of H. pylori (urease and arginase respectively). Our results strongly suggest that (i) the H. pylori paralogous amidases have evolved to achieve enzymatic specialization after ancestral gene duplication; and (ii) the production of these enzymes is regulated to maintain intracellular nitrogen balance in H. pylori." @default.
• W2109556475 created "2016-06-24" @default.
• W2109556475 creator A5017518614 @default.
• W2109556475 creator A5085199338 @default.
• W2109556475 creator A5087191671 @default.
• W2109556475 date "2001-05-01" @default.
• W2109556475 modified "2023-10-17" @default.
• W2109556475 title "The AmiE aliphatic amidase and AmiF formamidase of Helicobacter pylori: natural evolution of two enzyme paralogues" @default.
• W2109556475 cites W1176872883 @default.
• W2109556475 cites W1554191921 @default.
• W2109556475 cites W1729731710 @default.
• W2109556475 cites W1894613439 @default.
• W2109556475 cites W1911278511 @default.
• W2109556475 cites W1943163399 @default.
• W2109556475 cites W1965182355 @default.
• W2109556475 cites W1970745550 @default.
• W2109556475 cites W1975965973 @default.
• W2109556475 cites W1982274454 @default.
• W2109556475 cites W1983151151 @default.
• W2109556475 cites W1984640298 @default.
• W2109556475 cites W1987213864 @default.
• W2109556475 cites W1997581572 @default.
• W2109556475 cites W1999752983 @default.
• W2109556475 cites W2009157046 @default.
• W2109556475 cites W2010856921 @default.
• W2109556475 cites W2013458312 @default.
• W2109556475 cites W2014560396 @default.
• W2109556475 cites W2016299826 @default.
• W2109556475 cites W2028622989 @default.
• W2109556475 cites W2030797949 @default.
• W2109556475 cites W2033330055 @default.
• W2109556475 cites W2034337895 @default.
• W2109556475 cites W2034408556 @default.
• W2109556475 cites W2035291650 @default.
• W2109556475 cites W2039731543 @default.
• W2109556475 cites W2044320282 @default.
• W2109556475 cites W2045284256 @default.
• W2109556475 cites W2059135418 @default.
• W2109556475 cites W2065512356 @default.
• W2109556475 cites W2076970523 @default.
• W2109556475 cites W2088092681 @default.
• W2109556475 cites W2088436037 @default.
• W2109556475 cites W2093352621 @default.
• W2109556475 cites W2099768877 @default.
• W2109556475 cites W2099871694 @default.
• W2109556475 cites W2105820571 @default.
• W2109556475 cites W2110132014 @default.
• W2109556475 cites W2114619963 @default.
• W2109556475 cites W2116531314 @default.
• W2109556475 cites W2124539031 @default.
• W2109556475 cites W2134153749 @default.
• W2109556475 cites W2137815990 @default.
• W2109556475 cites W2143107875 @default.
• W2109556475 cites W2159266221 @default.
• W2109556475 cites W2167973356 @default.
• W2109556475 cites W2168610740 @default.
• W2109556475 cites W2223384704 @default.
• W2109556475 cites W4234541047 @default.
• W2109556475 cites W4250267240 @default.
• W2109556475 cites W4252172606 @default.
• W2109556475 cites W4254246059 @default.
• W2109556475 cites W7447920 @default.
• W2109556475 doi "https://doi.org/10.1046/j.1365-2958.2001.02400.x" @default.
• W2109556475 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11359566" @default.
• W2109556475 hasPublicationYear "2001" @default.
• W2109556475 type Work @default.
• W2109556475 sameAs 2109556475 @default.
• W2109556475 citedByCount "74" @default.
• W2109556475 countsByYear W21095564752012 @default.
• W2109556475 countsByYear W21095564752013 @default.
• W2109556475 countsByYear W21095564752014 @default.
• W2109556475 countsByYear W21095564752015 @default.
• W2109556475 countsByYear W21095564752016 @default.
• W2109556475 countsByYear W21095564752017 @default.
• W2109556475 countsByYear W21095564752018 @default.
• W2109556475 countsByYear W21095564752019 @default.
• W2109556475 countsByYear W21095564752020 @default.
• W2109556475 countsByYear W21095564752021 @default.
• W2109556475 countsByYear W21095564752022 @default.
• W2109556475 countsByYear W21095564752023 @default.
• W2109556475 crossrefType "journal-article" @default.
• W2109556475 hasAuthorship W2109556475A5017518614 @default.
• W2109556475 hasAuthorship W2109556475A5085199338 @default.
• W2109556475 hasAuthorship W2109556475A5087191671 @default.
• W2109556475 hasConcept C181199279 @default.
• W2109556475 hasConcept C2778270854 @default.
• W2109556475 hasConcept C54355233 @default.
• W2109556475 hasConcept C55493867 @default.
• W2109556475 hasConcept C65588867 @default.
• W2109556475 hasConcept C86803240 @default.
• W2109556475 hasConcept C94412978 @default.
• W2109556475 hasConceptScore W2109556475C181199279 @default.
• W2109556475 hasConceptScore W2109556475C2778270854 @default.
• W2109556475 hasConceptScore W2109556475C54355233 @default.
• W2109556475 hasConceptScore W2109556475C55493867 @default.
• W2109556475 hasConceptScore W2109556475C65588867 @default.
• W2109556475 hasConceptScore W2109556475C86803240 @default.
• W2109556475 hasConceptScore W2109556475C94412978 @default.

• next