Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109571812> ?p ?o ?g. }
- W2109571812 endingPage "3619" @default.
- W2109571812 startingPage "3604" @default.
- W2109571812 abstract "TGFβ is a potent regulator of cell differentiation in many cell types. On aortic endothelial cells, TGFβ1 displays angiogenic properties in inducing capillary-like tube formation in collagen I gels, in vitro. We investigated cytoskeletal changes that precede tube formation and related these alterations to the effects of TGFβ1 on the activation state of members of the RhoGTPase family. TGFβ1 promotes cell elongation and stress fiber formation in aortic endothelial cells. Using cell lines with inducible expression of Rac1 mutants, we show that these events are mimicked by expression of dominant-negative Rac1 whereas the constitutively active mutant prevents the TGFβ1-mediated change of phenotype. Although TGFβ1 induces an initial rise in the Rac1-GTP content, this phase is followed by a prolonged loss of the active form. In contrast, RhoA activity increases progressively and reaches a plateau when Rac1-GTP is no longer detectable. Prolonged inhibition of Rac1 appears necessary and sufficient for the increase in RhoA-GTP. In situ examination of Rho activity in TGFβ1-treated cells provides evidence that active RhoA relocalizes to the tips of elongated cells. Inhibiting the Rho effector ROCK abrogates tube formation. Thus, Rac1 and RhoA are regulated by TGFβ1 in the process of endothelial tube formation in collagen I gels." @default.
- W2109571812 created "2016-06-24" @default.
- W2109571812 creator A5001297230 @default.
- W2109571812 creator A5007964369 @default.
- W2109571812 creator A5042463966 @default.
- W2109571812 creator A5050056073 @default.
- W2109571812 creator A5061596661 @default.
- W2109571812 creator A5073566280 @default.
- W2109571812 date "2006-11-01" @default.
- W2109571812 modified "2023-10-18" @default.
- W2109571812 title "TGFβ1-induced aortic endothelial morphogenesis requires signaling by small GTPases Rac1 and RhoA" @default.
- W2109571812 cites W139895096 @default.
- W2109571812 cites W1562397511 @default.
- W2109571812 cites W1574172480 @default.
- W2109571812 cites W1905551819 @default.
- W2109571812 cites W1953757484 @default.
- W2109571812 cites W1964513396 @default.
- W2109571812 cites W1965094170 @default.
- W2109571812 cites W1970017200 @default.
- W2109571812 cites W1970523400 @default.
- W2109571812 cites W1971018030 @default.
- W2109571812 cites W1972049776 @default.
- W2109571812 cites W1990655990 @default.
- W2109571812 cites W2000292756 @default.
- W2109571812 cites W2002244701 @default.
- W2109571812 cites W2008890007 @default.
- W2109571812 cites W2019520439 @default.
- W2109571812 cites W2021263701 @default.
- W2109571812 cites W2026325139 @default.
- W2109571812 cites W2027810531 @default.
- W2109571812 cites W2028220020 @default.
- W2109571812 cites W2033887719 @default.
- W2109571812 cites W2039787319 @default.
- W2109571812 cites W2041981359 @default.
- W2109571812 cites W2047453903 @default.
- W2109571812 cites W2052398489 @default.
- W2109571812 cites W2055519002 @default.
- W2109571812 cites W2062199188 @default.
- W2109571812 cites W2063420622 @default.
- W2109571812 cites W2063868306 @default.
- W2109571812 cites W2066926102 @default.
- W2109571812 cites W2069532196 @default.
- W2109571812 cites W2072743356 @default.
- W2109571812 cites W2074190587 @default.
- W2109571812 cites W2077603983 @default.
- W2109571812 cites W2077792958 @default.
- W2109571812 cites W2086801198 @default.
- W2109571812 cites W2093172971 @default.
- W2109571812 cites W2100537036 @default.
- W2109571812 cites W2106835270 @default.
- W2109571812 cites W2112634792 @default.
- W2109571812 cites W2116577936 @default.
- W2109571812 cites W2118650242 @default.
- W2109571812 cites W2121643037 @default.
- W2109571812 cites W2124739780 @default.
- W2109571812 cites W2132052155 @default.
- W2109571812 cites W2132208081 @default.
- W2109571812 cites W2134807132 @default.
- W2109571812 cites W2138229905 @default.
- W2109571812 cites W2141042173 @default.
- W2109571812 cites W2153111772 @default.
- W2109571812 cites W2162238950 @default.
- W2109571812 cites W2164321835 @default.
- W2109571812 cites W2166676058 @default.
- W2109571812 cites W2168476587 @default.
- W2109571812 cites W2768578212 @default.
- W2109571812 doi "https://doi.org/10.1016/j.yexcr.2006.08.006" @default.
- W2109571812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16978608" @default.
- W2109571812 hasPublicationYear "2006" @default.
- W2109571812 type Work @default.
- W2109571812 sameAs 2109571812 @default.
- W2109571812 citedByCount "24" @default.
- W2109571812 countsByYear W21095718122012 @default.
- W2109571812 countsByYear W21095718122014 @default.
- W2109571812 countsByYear W21095718122015 @default.
- W2109571812 countsByYear W21095718122016 @default.
- W2109571812 countsByYear W21095718122017 @default.
- W2109571812 countsByYear W21095718122020 @default.
- W2109571812 countsByYear W21095718122022 @default.
- W2109571812 countsByYear W21095718122023 @default.
- W2109571812 crossrefType "journal-article" @default.
- W2109571812 hasAuthorship W2109571812A5001297230 @default.
- W2109571812 hasAuthorship W2109571812A5007964369 @default.
- W2109571812 hasAuthorship W2109571812A5042463966 @default.
- W2109571812 hasAuthorship W2109571812A5050056073 @default.
- W2109571812 hasAuthorship W2109571812A5061596661 @default.
- W2109571812 hasAuthorship W2109571812A5073566280 @default.
- W2109571812 hasConcept C118131993 @default.
- W2109571812 hasConcept C123012128 @default.
- W2109571812 hasConcept C151166631 @default.
- W2109571812 hasConcept C202751555 @default.
- W2109571812 hasConcept C207332259 @default.
- W2109571812 hasConcept C2777093181 @default.
- W2109571812 hasConcept C2779717556 @default.
- W2109571812 hasConcept C2780040266 @default.
- W2109571812 hasConcept C2781099445 @default.
- W2109571812 hasConcept C2909331102 @default.
- W2109571812 hasConcept C55493867 @default.