FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109572223> ?p ?o ?g. }

• W2109572223 endingPage "51" @default.
• W2109572223 startingPage "2645" @default.
• W2109572223 abstract "In order to assess prostatic tissue as a target for receptor-mediated estrogen action, we have examined the regulation of estrogen (ER) and progestin receptors (PgR) by estrogen, antiestrogen, and progesterone in cytosolic and nuclear fractions of the R3327H (Dunning) prostatic adenocarcinoma of the rat. Twenty micrograms diethylstilbestrol (DES) with or without 800 micrograms tamoxifen (Tam) were injected s.c. in oil 5 times weekly for 2 weeks. Controls were given oil only. Estrogen receptor assays were carried out using [3H]estradiol and a hydroxylapatite exchange method. Progestin receptors were assayed using [3H]R5020 and dextran-coated charcoal to separate free and bound steroid. All binding data were evaluated by using Scatchard analysis. Treatment with DES depleted cytosolic ER, promoted association of ER with the nuclear fraction, and concomitantly increased PgR concentrations in amounts proportional to nuclear ER. Treatment with Tam alone resulted in higher nuclear ER concentrations than treatment with DES, but induced only one-fifth the amount of PgR. Treatment with DES plus Tam resulted in similar nuclear ER concentrations as with Tam alone, but PgR concentrations were intermediate between those observed with DES alone and Tam alone. Thus Tam exhibited both estrogenic and antiestrogenic properties. In this experiment, the same cytosolic and nuclear extracts were also assayed for ER by using monoclonal antibodies to the receptor in an enzyme immunoassay. No significant differences were observed between the results obtained by the radioligand and enzyme immunoassay methods in the cytosol and nuclear fractions from the control and DES-treated tumors. However in both Tam-treated groups, the ER values obtained by the enzyme immunoassay method were significantly higher than those obtained by the radioligand method in both cytosolic and nuclear fractions. This confirms the observations made by others in female target organs, that monoclonal antibody to ER reacts differently with the Tam-bound ER complex than with the estradiol-bound ER complex. In a separate experiment, administration of progesterone with DES decreased the concentration of nuclear ER to less than one-half that observed after administration of DES alone, with proportional decreases in both cytosolic and nuclear PgR. All these observations indicate that the control of ER and PgR concentrations in this prostatic tumor is identical to that observed in female rat target organs. Use of an immunohistochemical method for the detection of ER in frozen sections indicated that the receptor was localized in the glandular epithelium in both control and DES-treated tumors." @default.
• W2109572223 created "2016-06-24" @default.
• W2109572223 creator A5003280621 @default.
• W2109572223 creator A5009362088 @default.
• W2109572223 creator A5032568672 @default.
• W2109572223 creator A5061293989 @default.
• W2109572223 creator A5074069088 @default.
• W2109572223 date "1987-05-15" @default.
• W2109572223 modified "2023-10-03" @default.
• W2109572223 title "Regulation of estrogen and progestin receptor concentrations in an experimental rat prostatic carcinoma by estrogen, antiestrogen, and progesterone." @default.
• W2109572223 cites W1489626592 @default.
• W2109572223 cites W1513449169 @default.
• W2109572223 cites W1514766530 @default.
• W2109572223 cites W1574037425 @default.
• W2109572223 cites W1575646106 @default.
• W2109572223 cites W1775749144 @default.
• W2109572223 cites W1793453977 @default.
• W2109572223 cites W1964482095 @default.
• W2109572223 cites W1966361420 @default.
• W2109572223 cites W1966408814 @default.
• W2109572223 cites W1968119599 @default.
• W2109572223 cites W1972910332 @default.
• W2109572223 cites W1976495127 @default.
• W2109572223 cites W1977189077 @default.
• W2109572223 cites W1980000227 @default.
• W2109572223 cites W1983519421 @default.
• W2109572223 cites W1983649348 @default.
• W2109572223 cites W1989080765 @default.
• W2109572223 cites W1990520988 @default.
• W2109572223 cites W2011321576 @default.
• W2109572223 cites W2018201103 @default.
• W2109572223 cites W2018560313 @default.
• W2109572223 cites W2026971413 @default.
• W2109572223 cites W2038022884 @default.
• W2109572223 cites W2045272284 @default.
• W2109572223 cites W2050279398 @default.
• W2109572223 cites W2074175987 @default.
• W2109572223 cites W2083973655 @default.
• W2109572223 cites W2084873143 @default.
• W2109572223 cites W2087045037 @default.
• W2109572223 cites W2087326277 @default.
• W2109572223 cites W2103798082 @default.
• W2109572223 cites W2115075553 @default.
• W2109572223 cites W2154831507 @default.
• W2109572223 cites W2158701446 @default.
• W2109572223 cites W2166657294 @default.
• W2109572223 cites W2409929183 @default.
• W2109572223 cites W2417648653 @default.
• W2109572223 cites W2497472514 @default.
• W2109572223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3552202" @default.
• W2109572223 hasPublicationYear "1987" @default.
• W2109572223 type Work @default.
• W2109572223 sameAs 2109572223 @default.
• W2109572223 citedByCount "2" @default.
• W2109572223 countsByYear W21095722232016 @default.
• W2109572223 crossrefType "journal-article" @default.
• W2109572223 hasAuthorship W2109572223A5003280621 @default.
• W2109572223 hasAuthorship W2109572223A5009362088 @default.
• W2109572223 hasAuthorship W2109572223A5032568672 @default.
• W2109572223 hasAuthorship W2109572223A5061293989 @default.
• W2109572223 hasAuthorship W2109572223A5074069088 @default.
• W2109572223 hasConcept C121608353 @default.
• W2109572223 hasConcept C126322002 @default.
• W2109572223 hasConcept C134018914 @default.
• W2109572223 hasConcept C185592680 @default.
• W2109572223 hasConcept C2776067312 @default.
• W2109572223 hasConcept C2776607906 @default.
• W2109572223 hasConcept C2777164284 @default.
• W2109572223 hasConcept C2777176818 @default.
• W2109572223 hasConcept C2778656385 @default.
• W2109572223 hasConcept C2781397067 @default.
• W2109572223 hasConcept C530470458 @default.
• W2109572223 hasConcept C71924100 @default.
• W2109572223 hasConcept C84606932 @default.
• W2109572223 hasConcept C86803240 @default.
• W2109572223 hasConcept C96307122 @default.
• W2109572223 hasConceptScore W2109572223C121608353 @default.
• W2109572223 hasConceptScore W2109572223C126322002 @default.
• W2109572223 hasConceptScore W2109572223C134018914 @default.
• W2109572223 hasConceptScore W2109572223C185592680 @default.
• W2109572223 hasConceptScore W2109572223C2776067312 @default.
• W2109572223 hasConceptScore W2109572223C2776607906 @default.
• W2109572223 hasConceptScore W2109572223C2777164284 @default.
• W2109572223 hasConceptScore W2109572223C2777176818 @default.
• W2109572223 hasConceptScore W2109572223C2778656385 @default.
• W2109572223 hasConceptScore W2109572223C2781397067 @default.
• W2109572223 hasConceptScore W2109572223C530470458 @default.
• W2109572223 hasConceptScore W2109572223C71924100 @default.
• W2109572223 hasConceptScore W2109572223C84606932 @default.
• W2109572223 hasConceptScore W2109572223C86803240 @default.
• W2109572223 hasConceptScore W2109572223C96307122 @default.
• W2109572223 hasIssue "10" @default.
• W2109572223 hasLocation W21095722231 @default.
• W2109572223 hasOpenAccess W2109572223 @default.
• W2109572223 hasPrimaryLocation W21095722231 @default.
• W2109572223 hasRelatedWork W1574037425 @default.
• W2109572223 hasRelatedWork W1621741447 @default.
• W2109572223 hasRelatedWork W1982981718 @default.

• next