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• W2109597966 abstract "We have recently described the existence of a chaperone activity for the dimeric peptidyl-prolyl cis/trans isomerase FkpA from the periplasm of Escherichia coli that is independent of its isomerase activity. We have now investigated the molecular mechanism of these two activities in vitro in greater detail. The isomerase activity with a protein substrate (RNaseT1) is characterized by a 100-fold higher k(cat)/K(M) value than with a short tetrapeptide substrate. This enhanced activity with a protein is due to an increased affinity towards the protein substrate mediated by a polypeptide-binding site that is distinct from the active site. The chaperone activity is also mediated by interaction of folding and unfolding intermediates with a binding site that is most likely identical to the polypeptide-binding site which enhances catalysis. Both activities are thus mechanistically related, being based on the transient interaction with this high-affinity polypeptide-binding site. Only the isomerase activity, but not the chaperone activity, with the substrate citrate synthase can be inhibited by FK520. Experiments with the isolated domains of FkpA imply that both the isomerase and the chaperone site are located on the highly conserved FKBP domain. The additional amino-terminal domain mediates the dimerization and thus places the two active sites of the FKBP domains in juxtaposition, such that they can simultaneously interact with a protein, and this is required for full catalytic activity." @default.
• W2109597966 created "2016-06-24" @default.
• W2109597966 creator A5075641407 @default.
• W2109597966 creator A5083658123 @default.
• W2109597966 date "2001-07-01" @default.
• W2109597966 modified "2023-10-13" @default.
• W2109597966 title "High enzymatic activity and chaperone function are mechanistically related features of the dimeric E. coli peptidyl-prolyl-isomerase FkpA" @default.
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• W2109597966 doi "https://doi.org/10.1006/jmbi.2001.4747" @default.
• W2109597966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11428902" @default.
• W2109597966 hasPublicationYear "2001" @default.
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