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• W2109608997 abstract "We developed a rapid and reliable technique for specifically staining intact chloroplasts using the fluorescent dye carboxyfluorescein diacetate. Intact, import-competent chloroplasts were isolated simply and rapidly from soil-grown Arabidopsis thaliana plants, with yields of 20 ± 5 μg chlorophyll per g FW, greater than previously reported yields from soil-grown Arabidopsis. Traditional chloroplast isolation buffers sometimes contain low concentrations (<10 mM) sodium ascorbate as a general-purpose anti-oxidant, but we found that only Arabidopsis chloroplasts isolated in the presence of high concentrations (50–100 mM) of sodium ascorbate in the initial grinding buffer were import-competent." @default.
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• W2109608997 date "2004-05-01" @default.
• W2109608997 modified "2023-09-23" @default.
• W2109608997 title "Uptake of a Fluorescent Dye as a Swift and Simple Indicator of Organelle Intactness: Import-competent Chloroplasts from Soil-grown <i>Arabidopsis</i>" @default.
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• W2109608997 doi "https://doi.org/10.1177/002215540405200514" @default.
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