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- W2109623486 abstract "Gene silencing mediated by RNA interference (RNAi) was first discovered in Caenorhabditis elegans, and was subsequently recognized in various other organisms. In mammalian cells, RNAi can be induced by small interfering RNAs (siRNAs). In earlier studies, our group developed a vector‐based system for expression of siRNA under control of a polymerase III promoter, the U6 promoter, which can induce RNAi in living cells. We here describe a system for controlling the U6 promoter‐driven expression of siRNA using the Cre–loxP recombination system. We constructed a ‘Cre‐On’ siRNA expression vector which could be switched on upon excision catalyzed by Cre recombinase, which was delivered to cells directly from the medium as a fusion protein. An examination of the effectiveness of RNAi against a reporter gene revealed that addition of TAT‐NLS‐Cre (where NLS is a nuclear localization signal and TAT is a peptide of 11 amino acids derived from HIV) to the medium resulted in plasmid recombination, generation of siRNA and suppression of reporter activity. This system should allow us to induce RNAi in a spatially, temporally, cell type‐specifically or tissue‐specifically controlled manner and potentiate the improved application of RNAi in both an experimental and a therapeutic context." @default.
- W2109623486 created "2016-06-24" @default.
- W2109623486 creator A5046213298 @default.
- W2109623486 creator A5048761862 @default.
- W2109623486 creator A5059765912 @default.
- W2109623486 date "2004-04-19" @default.
- W2109623486 modified "2023-10-16" @default.
- W2109623486 title "Control of siRNA expression using the Cre-loxP recombination system" @default.
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- W2109623486 doi "https://doi.org/10.1093/nar/gnh061" @default.
- W2109623486 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/407841" @default.
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