FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109642110> ?p ?o ?g. }

• W2109642110 endingPage "3195" @default.
• W2109642110 startingPage "3188" @default.
• W2109642110 abstract "Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies that more accurately reflect patient-specific in vivo tissue microenvironments at the time of staining. However, current tissue processing techniques involve several sequential stages with concomitant cell losses, and as such are inappropriate for use with small biopsies. Accordingly, we present a simple method for combined antibody-labelling and dissociation of heterogeneous cells from a tumour mass, which reduces the number of processing steps. Perfusion of ex vivo tissue at 4°C with antibodies and enzymes slows cellular activity while allowing sufficient time for the diffusion of minimally active enzymes. In situ antibody-labelled cells are then dissociated at 37°C from the tumour mass, whereupon hydrogel-filled channels allow the release of relatively low cell numbers (<1000) into a biomimetic microenvironment. This novel approach to sample processing is then further integrated with hydrogel-based electrokinetic transport of the freshly liberated fluorescent cells for downstream detection. It is anticipated that this integrated microfluidic methodology will have wide-ranging biomedical and clinical applications." @default.
• W2109642110 created "2016-06-24" @default.
• W2109642110 creator A5011254889 @default.
• W2109642110 creator A5017413865 @default.
• W2109642110 creator A5033891877 @default.
• W2109642110 creator A5034494601 @default.
• W2109642110 creator A5053179093 @default.
• W2109642110 date "2011-10-25" @default.
• W2109642110 modified "2023-09-26" @default.
• W2109642110 title "On-chip integrated labelling, transport and detection of tumour cells" @default.
• W2109642110 cites W1965001670 @default.
• W2109642110 cites W1968830734 @default.
• W2109642110 cites W1968836546 @default.
• W2109642110 cites W1976671976 @default.
• W2109642110 cites W1993221076 @default.
• W2109642110 cites W1993426721 @default.
• W2109642110 cites W1999947472 @default.
• W2109642110 cites W2017656225 @default.
• W2109642110 cites W2019117408 @default.
• W2109642110 cites W2029962960 @default.
• W2109642110 cites W2030976820 @default.
• W2109642110 cites W2031716651 @default.
• W2109642110 cites W2033870293 @default.
• W2109642110 cites W2035991148 @default.
• W2109642110 cites W2036584424 @default.
• W2109642110 cites W2037248548 @default.
• W2109642110 cites W2044499660 @default.
• W2109642110 cites W2045344646 @default.
• W2109642110 cites W2050548793 @default.
• W2109642110 cites W2050737292 @default.
• W2109642110 cites W2052862499 @default.
• W2109642110 cites W2057360104 @default.
• W2109642110 cites W2058368262 @default.
• W2109642110 cites W2061629486 @default.
• W2109642110 cites W2061708936 @default.
• W2109642110 cites W2062265555 @default.
• W2109642110 cites W2075116234 @default.
• W2109642110 cites W2075231036 @default.
• W2109642110 cites W2084863505 @default.
• W2109642110 cites W2089706661 @default.
• W2109642110 cites W2119339939 @default.
• W2109642110 cites W2124611535 @default.
• W2109642110 cites W2133762858 @default.
• W2109642110 cites W2137484390 @default.
• W2109642110 cites W2142700516 @default.
• W2109642110 cites W2153617101 @default.
• W2109642110 cites W2154976881 @default.
• W2109642110 cites W2157506064 @default.
• W2109642110 cites W2323966097 @default.
• W2109642110 cites W2338690157 @default.
• W2109642110 cites W4239637714 @default.
• W2109642110 cites W4256241916 @default.
• W2109642110 cites W97377992 @default.
• W2109642110 doi "https://doi.org/10.1002/elps.201100172" @default.
• W2109642110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22025027" @default.
• W2109642110 hasPublicationYear "2011" @default.
• W2109642110 type Work @default.
• W2109642110 sameAs 2109642110 @default.
• W2109642110 citedByCount "6" @default.
• W2109642110 countsByYear W21096421102013 @default.
• W2109642110 countsByYear W21096421102014 @default.
• W2109642110 countsByYear W21096421102016 @default.
• W2109642110 countsByYear W21096421102017 @default.
• W2109642110 crossrefType "journal-article" @default.
• W2109642110 hasAuthorship W2109642110A5011254889 @default.
• W2109642110 hasAuthorship W2109642110A5017413865 @default.
• W2109642110 hasAuthorship W2109642110A5033891877 @default.
• W2109642110 hasAuthorship W2109642110A5034494601 @default.
• W2109642110 hasAuthorship W2109642110A5053179093 @default.
• W2109642110 hasConcept C104317684 @default.
• W2109642110 hasConcept C12554922 @default.
• W2109642110 hasConcept C127413603 @default.
• W2109642110 hasConcept C127716648 @default.
• W2109642110 hasConcept C136229726 @default.
• W2109642110 hasConcept C150903083 @default.
• W2109642110 hasConcept C153911025 @default.
• W2109642110 hasConcept C159654299 @default.
• W2109642110 hasConcept C171250308 @default.
• W2109642110 hasConcept C185592680 @default.
• W2109642110 hasConcept C192562407 @default.
• W2109642110 hasConcept C202751555 @default.
• W2109642110 hasConcept C203014093 @default.
• W2109642110 hasConcept C207001950 @default.
• W2109642110 hasConcept C26291073 @default.
• W2109642110 hasConcept C2778015335 @default.
• W2109642110 hasConcept C2780523633 @default.
• W2109642110 hasConcept C2988198817 @default.
• W2109642110 hasConcept C2989209122 @default.
• W2109642110 hasConcept C542903549 @default.
• W2109642110 hasConcept C553184892 @default.
• W2109642110 hasConcept C55493867 @default.
• W2109642110 hasConcept C61696701 @default.
• W2109642110 hasConcept C71924100 @default.
• W2109642110 hasConcept C8673954 @default.
• W2109642110 hasConcept C86803240 @default.
• W2109642110 hasConceptScore W2109642110C104317684 @default.
• W2109642110 hasConceptScore W2109642110C12554922 @default.
• W2109642110 hasConceptScore W2109642110C127413603 @default.

• next