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- W2109654828 endingPage "886" @default.
- W2109654828 startingPage "867" @default.
- W2109654828 abstract "At the base of the bacterial flagella, a cytoplasmic rotor (the C-ring) generates torque and reverses rotation sense in response to stimuli. The bulk of the C-ring forms from many copies of the proteins FliG, FliM, and FliN, which together constitute the switch complex. To help resolve outstanding issues regarding C-ring architecture, we have investigated interactions between FliM and FliG from Thermotoga maritima with X-ray crystallography and pulsed dipolar ESR spectroscopy (PDS). A new crystal structure of an 11-unit FliG:FliM complex produces a large arc with a curvature consistent with the dimensions of the C-ring. Previously determined structures along with this new structure provided a basis to test switch complex assembly models. PDS combined with mutational studies and targeted cross-linking reveal that FliM and FliG interact through their middle domains to form both parallel and antiparallel arrangements in solution. Residue substitutions at predicted interfaces disrupt higher-order complexes that are primarily mediated by contacts between the C-terminal domain of FliG and the middle domain of a neighboring FliG molecule. Spin separations among multi-labeled components fit a self-consistent model that agree well with electron microscopy images of the C-ring. An activated form of the response regulator CheY destabilizes the parallel arrangement of FliM molecules to perturb FliG alignment in a process that may reflect the onset of rotation switching. These data suggest a model of C-ring assembly in which intermolecular contacts among FliG domains provide a template for FliM assembly and cooperative transitions." @default.
- W2109654828 created "2016-06-24" @default.
- W2109654828 creator A5006552242 @default.
- W2109654828 creator A5016966558 @default.
- W2109654828 creator A5043610144 @default.
- W2109654828 creator A5051828607 @default.
- W2109654828 creator A5061197349 @default.
- W2109654828 creator A5066929827 @default.
- W2109654828 creator A5071526287 @default.
- W2109654828 creator A5075535128 @default.
- W2109654828 date "2015-02-01" @default.
- W2109654828 modified "2023-10-17" @default.
- W2109654828 title "Assembly States of FliM and FliG within the Flagellar Switch Complex" @default.
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