FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109668833> ?p ?o ?g. }

• W2109668833 endingPage "319" @default.
• W2109668833 startingPage "313" @default.
• W2109668833 abstract "Summary Five strains of fungi belonging to Aspergillus sp. were evaluated by casein agar plate assay and a wheat bran-based solid-state fermentation for selecting a neutral protease-producing culture. Based on the results, A. oryzae NRRL 2217 was selected for further studies. Sixteen different agro-industrial residues were evaluated for their potential to serve as a substrate for neutral protease production by this fungal strain. Results showed that a combination of coconut oil cake and wheat bran in the mass ratio of 1:3 was the best substrate for enzyme production. Various process parameters influencing protease production including fermentation time, initial moisture content, and fermentation temperature were optimised. The medium was supplemented with different nutrients in the form of organic and inorganic nitrogen and carbon sources. Supplementation of chitin increased the enzyme production significantly. Ammonium nitrate as inorganic nitrogen supplement slightly enhanced enzyme production. No organic nitrogen supplement was effective enhancer of enzyme production. Fermentation was performed under optimised conditions (initial moisture content V/m = 50 %, temperature 30 °C, 48 h). Partial purification of the enzyme resulted in a 3-fold increase in the specific activity of the enzyme. The partially purified enzyme was characterised by various features that govern the enzyme activity such as assay temperature, assay pH and substrate concentration. The effect of various metal ions and known protease inhibitors on the enzyme activity was also studied. The enzyme was found to be stable in pH range 7.0–7.5, and at temperature of 50 °C for 35 min. By the activating effect of divalent cations (Mg 2+ ,C a 2+ ,F e 2+ ) and inhibiting effect of certain chelating agents (EGTA, EDTA), the enzyme was found to be a metalloprotease." @default.
• W2109668833 created "2016-06-24" @default.
• W2109668833 creator A5004887250 @default.
• W2109668833 creator A5039791172 @default.
• W2109668833 creator A5052797351 @default.
• W2109668833 creator A5057531103 @default.
• W2109668833 creator A5074744409 @default.
• W2109668833 date "2005-10-01" @default.
• W2109668833 modified "2023-10-02" @default.
• W2109668833 title "Production and Partial Purification of a Neutral Metalloprotease by Fungal Mixed Substrate Fermentation" @default.
• W2109668833 cites W1775749144 @default.
• W2109668833 cites W1965840544 @default.
• W2109668833 cites W1990761728 @default.
• W2109668833 cites W2001049522 @default.
• W2109668833 cites W2001490951 @default.
• W2109668833 cites W2008419295 @default.
• W2109668833 cites W2049789982 @default.
• W2109668833 cites W2054229854 @default.
• W2109668833 cites W2073815568 @default.
• W2109668833 cites W2076153011 @default.
• W2109668833 cites W2084661556 @default.
• W2109668833 cites W2102628698 @default.
• W2109668833 cites W2139481502 @default.
• W2109668833 cites W603517135 @default.
• W2109668833 hasPublicationYear "2005" @default.
• W2109668833 type Work @default.
• W2109668833 sameAs 2109668833 @default.
• W2109668833 citedByCount "30" @default.
• W2109668833 countsByYear W21096688332012 @default.
• W2109668833 countsByYear W21096688332013 @default.
• W2109668833 countsByYear W21096688332014 @default.
• W2109668833 countsByYear W21096688332015 @default.
• W2109668833 countsByYear W21096688332016 @default.
• W2109668833 countsByYear W21096688332017 @default.
• W2109668833 countsByYear W21096688332018 @default.
• W2109668833 countsByYear W21096688332021 @default.
• W2109668833 crossrefType "journal-article" @default.
• W2109668833 hasAuthorship W2109668833A5004887250 @default.
• W2109668833 hasAuthorship W2109668833A5039791172 @default.
• W2109668833 hasAuthorship W2109668833A5052797351 @default.
• W2109668833 hasAuthorship W2109668833A5057531103 @default.
• W2109668833 hasAuthorship W2109668833A5074744409 @default.
• W2109668833 hasConcept C100544194 @default.
• W2109668833 hasConcept C178790620 @default.
• W2109668833 hasConcept C181199279 @default.
• W2109668833 hasConcept C185592680 @default.
• W2109668833 hasConcept C18903297 @default.
• W2109668833 hasConcept C206139338 @default.
• W2109668833 hasConcept C2776142715 @default.
• W2109668833 hasConcept C2776714187 @default.
• W2109668833 hasConcept C2776873940 @default.
• W2109668833 hasConcept C2777289219 @default.
• W2109668833 hasConcept C2779942233 @default.
• W2109668833 hasConcept C2781204770 @default.
• W2109668833 hasConcept C28212737 @default.
• W2109668833 hasConcept C31903555 @default.
• W2109668833 hasConcept C43617362 @default.
• W2109668833 hasConcept C55493867 @default.
• W2109668833 hasConcept C86803240 @default.
• W2109668833 hasConceptScore W2109668833C100544194 @default.
• W2109668833 hasConceptScore W2109668833C178790620 @default.
• W2109668833 hasConceptScore W2109668833C181199279 @default.
• W2109668833 hasConceptScore W2109668833C185592680 @default.
• W2109668833 hasConceptScore W2109668833C18903297 @default.
• W2109668833 hasConceptScore W2109668833C206139338 @default.
• W2109668833 hasConceptScore W2109668833C2776142715 @default.
• W2109668833 hasConceptScore W2109668833C2776714187 @default.
• W2109668833 hasConceptScore W2109668833C2776873940 @default.
• W2109668833 hasConceptScore W2109668833C2777289219 @default.
• W2109668833 hasConceptScore W2109668833C2779942233 @default.
• W2109668833 hasConceptScore W2109668833C2781204770 @default.
• W2109668833 hasConceptScore W2109668833C28212737 @default.
• W2109668833 hasConceptScore W2109668833C31903555 @default.
• W2109668833 hasConceptScore W2109668833C43617362 @default.
• W2109668833 hasConceptScore W2109668833C55493867 @default.
• W2109668833 hasConceptScore W2109668833C86803240 @default.
• W2109668833 hasIssue "4" @default.
• W2109668833 hasLocation W21096688331 @default.
• W2109668833 hasOpenAccess W2109668833 @default.
• W2109668833 hasPrimaryLocation W21096688331 @default.
• W2109668833 hasRelatedWork W126109032 @default.
• W2109668833 hasRelatedWork W1775749144 @default.
• W2109668833 hasRelatedWork W1965840544 @default.
• W2109668833 hasRelatedWork W1996497477 @default.
• W2109668833 hasRelatedWork W1997234557 @default.
• W2109668833 hasRelatedWork W1998261106 @default.
• W2109668833 hasRelatedWork W2001049522 @default.
• W2109668833 hasRelatedWork W2001490951 @default.
• W2109668833 hasRelatedWork W2008419295 @default.
• W2109668833 hasRelatedWork W2009674410 @default.
• W2109668833 hasRelatedWork W2033033039 @default.
• W2109668833 hasRelatedWork W2077610870 @default.
• W2109668833 hasRelatedWork W2085798465 @default.
• W2109668833 hasRelatedWork W2095688454 @default.
• W2109668833 hasRelatedWork W2100837269 @default.
• W2109668833 hasRelatedWork W2101501929 @default.
• W2109668833 hasRelatedWork W2102628698 @default.
• W2109668833 hasRelatedWork W2111341540 @default.

• next