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• W2109728482 abstract "Stable isotope labeling with amino acids in cell culture (SILAC) has risen as a powerful quantification technique in mass spectrometry (MS)–based proteomics in classical and modified forms. Previously, SILAC was limited to cultured cells because of the requirement of active protein synthesis; however, in recent years, it was expanded to model organisms and tissue samples. Specifically, the super-SILAC technique uses a mixture of SILAC-labeled cells as a spike-in standard for accurate quantification of unlabeled samples, thereby enabling quantification of human tissue samples. Here, we highlight the recent developments in super-SILAC and its application to the study of clinical samples, secretomes, post-translational modifications and organelle proteomes. Finally, we propose super-SILAC as a robust and accurate method that can be commercialized and applied to basic and clinical research." @default.
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• W2109728482 date "2014-11-18" @default.
• W2109728482 modified "2023-10-16" @default.
• W2109728482 title "Super-SILAC: current trends and future perspectives" @default.
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• W2109728482 doi "https://doi.org/10.1586/14789450.2015.982538" @default.
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