FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109741608> ?p ?o ?g. }

• W2109741608 abstract "Abstract Background High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; however, such approaches are blind to RNAs with short or no poly(A) tails, leading to an incomplete view of the transcriptome. Another challenge of preparing RNA-Seq libraries is to preserve the strand information of the RNAs. Design Here, we describe a procedure for preparing RNA-Seq libraries from 1 to 4 μg total RNA without poly(A) selection. Our method combines the deoxyuridine triphosphate (dUTP)/uracil-DNA glycosylase (UDG) strategy to achieve strand specificity with AMPure XP magnetic beads to perform size selection. Together, these steps eliminate gel purification, allowing a library to be made in less than two days. We barcode each library during the final PCR amplification step, allowing several samples to be sequenced in a single lane without sacrificing read length. Libraries prepared using this protocol are compatible with Illumina GAII, GAIIx and HiSeq 2000 platforms. Discussion The RNA-Seq protocol described here yields strand-specific transcriptome libraries without poly(A) selection, which provide approximately 90% mappable sequences. Typically, more than 85% of mapped reads correspond to protein-coding genes and only 6% derive from non-coding RNAs. The protocol has been used to measure RNA transcript identity and abundance in tissues from flies, mice, rats, chickens, and frogs, demonstrating its general applicability." @default.
• W2109741608 created "2016-06-24" @default.
• W2109741608 creator A5020225652 @default.
• W2109741608 creator A5064772392 @default.
• W2109741608 creator A5065361552 @default.
• W2109741608 creator A5084369008 @default.
• W2109741608 date "2012-12-01" @default.
• W2109741608 modified "2023-10-05" @default.
• W2109741608 title "Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection" @default.
• W2109741608 cites W1850391599 @default.
• W2109741608 cites W1965175512 @default.
• W2109741608 cites W1973839814 @default.
• W2109741608 cites W1976973748 @default.
• W2109741608 cites W1981509058 @default.
• W2109741608 cites W1995187134 @default.
• W2109741608 cites W2004798712 @default.
• W2109741608 cites W2023500202 @default.
• W2109741608 cites W2049137252 @default.
• W2109741608 cites W2049424521 @default.
• W2109741608 cites W2058805572 @default.
• W2109741608 cites W2060842569 @default.
• W2109741608 cites W2092977521 @default.
• W2109741608 cites W2094257363 @default.
• W2109741608 cites W2097065948 @default.
• W2109741608 cites W2097337885 @default.
• W2109741608 cites W2100305481 @default.
• W2109741608 cites W2118526609 @default.
• W2109741608 cites W2152045787 @default.
• W2109741608 cites W2170551349 @default.
• W2109741608 cites W4233902414 @default.
• W2109741608 doi "https://doi.org/10.1186/1758-907x-3-9" @default.
• W2109741608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3552703" @default.
• W2109741608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23273270" @default.
• W2109741608 hasPublicationYear "2012" @default.
• W2109741608 type Work @default.
• W2109741608 sameAs 2109741608 @default.
• W2109741608 citedByCount "121" @default.
• W2109741608 countsByYear W21097416082013 @default.
• W2109741608 countsByYear W21097416082014 @default.
• W2109741608 countsByYear W21097416082015 @default.
• W2109741608 countsByYear W21097416082016 @default.
• W2109741608 countsByYear W21097416082017 @default.
• W2109741608 countsByYear W21097416082018 @default.
• W2109741608 countsByYear W21097416082019 @default.
• W2109741608 countsByYear W21097416082020 @default.
• W2109741608 countsByYear W21097416082021 @default.
• W2109741608 countsByYear W21097416082022 @default.
• W2109741608 countsByYear W21097416082023 @default.
• W2109741608 crossrefType "journal-article" @default.
• W2109741608 hasAuthorship W2109741608A5020225652 @default.
• W2109741608 hasAuthorship W2109741608A5064772392 @default.
• W2109741608 hasAuthorship W2109741608A5065361552 @default.
• W2109741608 hasAuthorship W2109741608A5084369008 @default.
• W2109741608 hasBestOaLocation W21097416081 @default.
• W2109741608 hasConcept C104317684 @default.
• W2109741608 hasConcept C106337198 @default.
• W2109741608 hasConcept C107397762 @default.
• W2109741608 hasConcept C132917006 @default.
• W2109741608 hasConcept C13575015 @default.
• W2109741608 hasConcept C141231307 @default.
• W2109741608 hasConcept C150194340 @default.
• W2109741608 hasConcept C162317418 @default.
• W2109741608 hasConcept C3017666073 @default.
• W2109741608 hasConcept C51679486 @default.
• W2109741608 hasConcept C54355233 @default.
• W2109741608 hasConcept C552990157 @default.
• W2109741608 hasConcept C67705224 @default.
• W2109741608 hasConcept C70721500 @default.
• W2109741608 hasConcept C86803240 @default.
• W2109741608 hasConceptScore W2109741608C104317684 @default.
• W2109741608 hasConceptScore W2109741608C106337198 @default.
• W2109741608 hasConceptScore W2109741608C107397762 @default.
• W2109741608 hasConceptScore W2109741608C132917006 @default.
• W2109741608 hasConceptScore W2109741608C13575015 @default.
• W2109741608 hasConceptScore W2109741608C141231307 @default.
• W2109741608 hasConceptScore W2109741608C150194340 @default.
• W2109741608 hasConceptScore W2109741608C162317418 @default.
• W2109741608 hasConceptScore W2109741608C3017666073 @default.
• W2109741608 hasConceptScore W2109741608C51679486 @default.
• W2109741608 hasConceptScore W2109741608C54355233 @default.
• W2109741608 hasConceptScore W2109741608C552990157 @default.
• W2109741608 hasConceptScore W2109741608C67705224 @default.
• W2109741608 hasConceptScore W2109741608C70721500 @default.
• W2109741608 hasConceptScore W2109741608C86803240 @default.
• W2109741608 hasIssue "1" @default.
• W2109741608 hasLocation W21097416081 @default.
• W2109741608 hasLocation W21097416082 @default.
• W2109741608 hasLocation W21097416083 @default.
• W2109741608 hasLocation W21097416084 @default.
• W2109741608 hasLocation W21097416085 @default.
• W2109741608 hasLocation W21097416086 @default.
• W2109741608 hasOpenAccess W2109741608 @default.
• W2109741608 hasPrimaryLocation W21097416081 @default.
• W2109741608 hasRelatedWork W147645354 @default.
• W2109741608 hasRelatedWork W1546288527 @default.
• W2109741608 hasRelatedWork W2024683563 @default.
• W2109741608 hasRelatedWork W2065635989 @default.
• W2109741608 hasRelatedWork W2090461073 @default.
• W2109741608 hasRelatedWork W2098357503 @default.
• W2109741608 hasRelatedWork W2335514198 @default.

• next