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• W2109743669 abstract "Summary Zarkov, I., 2007. Comparative evaluation of three serological diagnostic tests in a chicken model of experimental intratracheal infection with avian influenza virus A/DUCK/ BULGARIA/05 H6N2. Bulg. J. Vet. Med. , 10 , No 4, 257266. After experimental intratracheal infection of chickens with an avian influenza viral isolate A/duck/Bulgaria/05 H6N2 (0.2 mL), the potential of the haemagglutination inhibition test (HIT), the immunodiffusion test (IDT) and enzyme-linked immunosorbent assay (ELISA) for antibody detection was evaluated. The results evidenced that in birds, antibodies to haemagglutinin, precipitating antibodies and IgG antibodies, detectable with ELISA, were formed. Subtype-specific antibodies were detected in all infected chickens. Serum titres ranged between 1:4 and 1:128 with predomination of 1:64 titres (44.8 %). The mean arithmetic titre for the experiment was 1:56.1. Precipitins were found out in all infected chickens. The titres ranged from undiluted to 1:128 with prevailing of 1:2 titres (27.6%). By means of ELISA, 100% seropositive birds were detected by the 7 and 14 th day whereas the percentage on days 21 was 85.7% and 28 was 80%. S/P values were the highest by the 14 th day (up to 2.776). The sensitivity, specificity and test agreement was 100% for HIT and IDT and 93.1% for ELISA on the background of a specificity of 100 %. The comparison of the three tests showed a specificity of 100 % for IDT and HIT and 93.1% for ELISA, sensitivity of 100 % and test agreement of 97.4 % for ELISA. The data for detection of type-specific antigens showed 93.1 % sensitivity for IDT and ELISA, specificity of 100 % and test agreement of 97.4 %." @default.
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• W2109743669 title "COMPARATIVE EVALUATION OF THREE SEROLOGICAL DIAGNOSTIC TESTS IN A CHICKEN MODEL OF EXPERIMENTAL INTRATRACHEAL INFECTION WITH AVIAN INFLUENZA VIRUS A/DUCK/BULGARIA/05 H6N2" @default.
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