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• W2109776304 abstract "A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1T, was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 °C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H2 and CO2. The major cellular fatty acids were iso-C15 : 0, iso-C14 : 0 and C16 : 0 DMA. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1T could be placed in cluster III of the genus Clostridium, being closely related to type strains of Clostridium hungatei (96.6 % sequence similarity), Clostridium termitidis (96.2 %) and Clostridium papyrosolvens (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1T and its close relatives, this strain represents a novel species of the genus Clostridium, for which the name Clostridium sufflavum sp. nov. is proposed. The type strain is CDT-1T (=JCM 14807T=DSM 19573T)." @default.
• W2109776304 created "2016-06-24" @default.
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• W2109776304 date "2009-04-30" @default.
• W2109776304 modified "2023-10-12" @default.
• W2109776304 title "Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste" @default.
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• W2109776304 doi "https://doi.org/10.1099/ijs.0.001719-0" @default.
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