FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2109778592> ?p ?o ?g. }

• W2109778592 endingPage "7426" @default.
• W2109778592 startingPage "7418" @default.
• W2109778592 abstract "A chimeric flavivirus infectious cDNA was constructed by exchanging the premembrane (prM) and envelope (E) genes of the yellow fever virus vaccine strain 17D (YF17D) with the corresponding genes of Modoc virus (MOD). This latter virus belongs to the cluster of the not-known vector flaviviruses and is, unlike YF17D, neuroinvasive in SCID mice. Replication of in vitro-transcribed RNA from this chimeric flavivirus was shown by [(3)H]uridine labeling and RNA analysis. Expression of the MOD prM and E proteins was monitored by radioimmunoprecipitation and revealed that the MOD proteins were correctly and efficiently produced from the chimeric precursor protein. The MOD E protein was shown to be N-linked glycosylated, whereas prM, as predicted from the genome sequence, did not contain N-linked carbohydrates. In Vero cells, the chimeric virus replicated with a similar efficiency as the parental viruses, although it formed smaller plaques than YF17D and MOD. In SCID mice that had been infected intraperitoneally with the chimeric virus, the viral load increased steadily until death. The MOD/YF virus, like MOD from which it had acquired the prM and E structural proteins, but unlike YF, proved neuroinvasive in SCID mice. Animals developed neurological symptoms about 15 days after inoculation and died shortly thereafter. The distribution of MOD/YF RNA in the brain of infected mice was similar to that observed in MOD-infected mice. The observations provide compelling evidence that the determinants of neuroinvasiveness of flaviviruses are entirely located in the envelope proteins prM and E." @default.
• W2109778592 created "2016-06-24" @default.
• W2109778592 creator A5011461272 @default.
• W2109778592 creator A5028386801 @default.
• W2109778592 creator A5036496196 @default.
• W2109778592 creator A5036860979 @default.
• W2109778592 creator A5040662398 @default.
• W2109778592 creator A5056476810 @default.
• W2109778592 creator A5063359248 @default.
• W2109778592 creator A5089010495 @default.
• W2109778592 creator A5090665639 @default.
• W2109778592 date "2004-07-15" @default.
• W2109778592 modified "2023-10-02" @default.
• W2109778592 title "Exchanging the Yellow Fever Virus Envelope Proteins with Modoc Virus prM and E Proteins Results in a Chimeric Virus That Is Neuroinvasive in SCID Mice" @default.
• W2109778592 cites W1557330854 @default.
• W2109778592 cites W1569947224 @default.
• W2109778592 cites W1894021355 @default.
• W2109778592 cites W1915221427 @default.
• W2109778592 cites W1965957692 @default.
• W2109778592 cites W1967826883 @default.
• W2109778592 cites W1970057644 @default.
• W2109778592 cites W1973123733 @default.
• W2109778592 cites W1975797606 @default.
• W2109778592 cites W1981895261 @default.
• W2109778592 cites W1988376722 @default.
• W2109778592 cites W1989136785 @default.
• W2109778592 cites W2004688624 @default.
• W2109778592 cites W2016088681 @default.
• W2109778592 cites W2016338330 @default.
• W2109778592 cites W2025264028 @default.
• W2109778592 cites W2032362216 @default.
• W2109778592 cites W2038891761 @default.
• W2109778592 cites W2040786554 @default.
• W2109778592 cites W2048907346 @default.
• W2109778592 cites W2052686753 @default.
• W2109778592 cites W2062805581 @default.
• W2109778592 cites W2062828687 @default.
• W2109778592 cites W2065347535 @default.
• W2109778592 cites W2083730757 @default.
• W2109778592 cites W2086921489 @default.
• W2109778592 cites W2098985529 @default.
• W2109778592 cites W2101187202 @default.
• W2109778592 cites W2105945183 @default.
• W2109778592 cites W2106494184 @default.
• W2109778592 cites W2107922358 @default.
• W2109778592 cites W2109100918 @default.
• W2109778592 cites W2110658293 @default.
• W2109778592 cites W2117415323 @default.
• W2109778592 cites W2120771396 @default.
• W2109778592 cites W2129296891 @default.
• W2109778592 cites W2134850550 @default.
• W2109778592 cites W2142481964 @default.
• W2109778592 cites W2147217990 @default.
• W2109778592 cites W2152708819 @default.
• W2109778592 cites W2155244918 @default.
• W2109778592 cites W2155660565 @default.
• W2109778592 cites W2163412375 @default.
• W2109778592 cites W2163478597 @default.
• W2109778592 cites W2169316634 @default.
• W2109778592 cites W2175522297 @default.
• W2109778592 cites W2186416474 @default.
• W2109778592 cites W2417056312 @default.
• W2109778592 cites W4235884550 @default.
• W2109778592 doi "https://doi.org/10.1128/jvi.78.14.7418-7426.2004" @default.
• W2109778592 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/434118" @default.
• W2109778592 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15220415" @default.
• W2109778592 hasPublicationYear "2004" @default.
• W2109778592 type Work @default.
• W2109778592 sameAs 2109778592 @default.
• W2109778592 citedByCount "28" @default.
• W2109778592 countsByYear W21097785922012 @default.
• W2109778592 countsByYear W21097785922013 @default.
• W2109778592 countsByYear W21097785922014 @default.
• W2109778592 countsByYear W21097785922015 @default.
• W2109778592 countsByYear W21097785922016 @default.
• W2109778592 countsByYear W21097785922017 @default.
• W2109778592 countsByYear W21097785922018 @default.
• W2109778592 countsByYear W21097785922019 @default.
• W2109778592 countsByYear W21097785922021 @default.
• W2109778592 countsByYear W21097785922023 @default.
• W2109778592 crossrefType "journal-article" @default.
• W2109778592 hasAuthorship W2109778592A5011461272 @default.
• W2109778592 hasAuthorship W2109778592A5028386801 @default.
• W2109778592 hasAuthorship W2109778592A5036496196 @default.
• W2109778592 hasAuthorship W2109778592A5036860979 @default.
• W2109778592 hasAuthorship W2109778592A5040662398 @default.
• W2109778592 hasAuthorship W2109778592A5056476810 @default.
• W2109778592 hasAuthorship W2109778592A5063359248 @default.
• W2109778592 hasAuthorship W2109778592A5089010495 @default.
• W2109778592 hasAuthorship W2109778592A5090665639 @default.
• W2109778592 hasBestOaLocation W21097785921 @default.
• W2109778592 hasConcept C104317684 @default.
• W2109778592 hasConcept C153911025 @default.
• W2109778592 hasConcept C159047783 @default.
• W2109778592 hasConcept C2522874641 @default.
• W2109778592 hasConcept C2779755945 @default.
• W2109778592 hasConcept C2780568436 @default.
• W2109778592 hasConcept C54355233 @default.

• next