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• W2109809933 abstract "In Saccharomyces cerevisiae, TFIID and SAGA principally mediate transcription of constitutive housekeeping genes and stress-inducible genes, respectively, by delivering TBP to the core promoter. Both are multi-protein complexes composed of 15 and 20 subunits, respectively, five of which are common and which may constitute a core sub-module in each complex. Although genome-wide gene expression studies have been conducted extensively in several TFIID and/or SAGA mutants, there are only a limited number of studies investigating genome-wide localization of the components of these two complexes. Specifically, there are no previous reports on localization of a complete set of Tafs and the effects of taf mutations on localization. Here, we examine the localization profiles of a complete set of Tafs, Gcn5, Bur6/Ncb2, Sua7, Tfa2, Tfg1, Tfb3 and Rpb1, on chromosomes III, IV and V by chromatin immunoprecipitation (ChIP)-chip analysis in wild-type and taf1-T657K mutant strains. In addition, we conducted conventional and sequential ChIP analysis of several ribosomal protein genes (RPGs) and non-RPGs. Intriguingly, the results revealed a novel relationship between TFIIB and NC2, simultaneous co-localization of SAGA and TFIID on RPG promoters, specific effects of taf1 mutation on Taf2 occupancy, and an indirect evidence for the existence of different TFIID conformations." @default.
• W2109809933 created "2016-06-24" @default.
• W2109809933 creator A5021309878 @default.
• W2109809933 creator A5048122665 @default.
• W2109809933 creator A5065556470 @default.
• W2109809933 creator A5069861564 @default.
• W2109809933 date "2009-12-21" @default.
• W2109809933 modified "2023-09-26" @default.
• W2109809933 title "Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters" @default.
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• W2109809933 doi "https://doi.org/10.1093/nar/gkp1172" @default.
• W2109809933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2847235" @default.
• W2109809933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20026583" @default.

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