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• W2109940718 startingPage "667" @default.
• W2109940718 abstract "A nuclear progestogen receptor was identified in the ovary of the spotted seatrout, Cynoscion nebulosus. A single class of high-affinity, low-capacity cytoplasmic binding sites for 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P) was characterized by saturation and Scatchard analyses (KD = 1.89 ± 0.61 nM, Bmax = 1.80 ± 0.63 pmol/g ovary, n = 4), as well as by one-point assay (Bmax = 1.41 ± 0.26 pmol/g ovary, n = 12). Analysis of the binding kinetics indicated a fairly rapid association rate (T1/2 = 72 ± 10.2 min) and a slightly slower dissociation rate (T1/2 = 99 ± 9.4 min). Competition studies revealed that several steroids exhibited the same range of affinity (17α, 20 β-P > 17α,20β,21-trihydroxy-4-pregnen-3-one (20β-S) > 11-deoxycorticosterone > progesterone) while others displayed an order of magnitude less affinity (17α-hydroxy-4-pregnen-3-one > pregnenolone > 11-deoxycortisol > testosterone). No displacement was found with 1000-fold excess estradiol-17β or cortisol. Binding activity was also present within the testis, but not in the brain, gill, muscle, or plasma. Nuclear binding was detected by DNA-cellulose column chromatography and was inhibited by the addition of molybdate, a characteristic of nuclear steroid receptors. Isolation and analysis of the nuclear fraction revealed specific binding with an order of magnitude lower affinity (KD = 20.7 ± 5.0 nM, n = 6) and capacity (0.22 ± 0.03 pmol/g ovary, n = 6), and steroid specificity nearly identical to that of the cytoplasmic receptor, although estradiol-17β competed for the nuclear binding sites (relative binding affinity ≈ 1% )." @default.
• W2109940718 created "2016-06-24" @default.
• W2109940718 creator A5029049466 @default.
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• W2109940718 date "1995-03-01" @default.
• W2109940718 modified "2023-10-14" @default.
• W2109940718 title "Characterization of a Progestogen Receptor in the Ovary of the Spotted Seatrout, Cynoscion Nebulosus1" @default.
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• W2109940718 doi "https://doi.org/10.1095/biolreprod52.3.667" @default.
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